|
|
| (One intermediate revision by the same user not shown) |
| Line 3: |
Line 3: |
| <StructureSection load='4hl0' size='340' side='right'caption='[[4hl0]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4hl0' size='340' side='right'caption='[[4hl0]], [[Resolution|resolution]] 2.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4hl0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Parasitic_roundworm Parasitic roundworm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HL0 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4hl0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxascaris_leonina Toxascaris leonina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HL0 FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nv1|3nv1]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hl0 OCA], [http://pdbe.org/4hl0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hl0 RCSB], [http://www.ebi.ac.uk/pdbsum/4hl0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hl0 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hl0 OCA], [https://pdbe.org/4hl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hl0 RCSB], [https://www.ebi.ac.uk/pdbsum/4hl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hl0 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/F1KZZ8_ASCSU F1KZZ8_ASCSU] |
| The full-length crystal structure of Toxascaris leonine galectin (Tl-gal), a galectin-9 homologue protein, was determined at a resolution of 2.0 A. Galectin-9 exhibits a variety of biological functions, including cell aggregation, eosinophil chemoattraction, activation and apoptosis of murine thymocytes, T cells and human melanoma cells. Similar to this galectin, Tl-gal may function as a regulatory molecule in the host immune system; however, no molecular or structural information has been reported for Tl-gal. Moreover, until now, there have been no reports of a full-length galectin structure. There are two molecules of Tl-gal per asymmetric unit in space group P2(1)2(1)2(1), and the N-terminal and C-terminal carbohydrate-recognition domains (NCRD and CCRD) of Tl-gal are composed of six-stranded beta-sheets and five-stranded beta-sheets with a short alpha-helix. The NCRD of Tl-gal resembles that of human galectin-7 and its CCRD resembles human galectin-9, but the residues in the interface and loop regions of the NCRD and CCRD are flexible and are related to interaction. Engagement of the T-cell immunoglobulin mucin-3 (Tim-3) immunoglobulin variable (IgV) domain by a galectin-9 ligand is known to be important for appropriate termination of T-helper 1 immune responses. To investigate the binding site of Tl-gal, the interaction between Tl-gal and Tim-3 was modelled. Tim-3 is docked into a major groove of the Tl-gal structure, which is larger and deeper than the minor groove. The structural information presented here will provide insight into the development of novel anti-inflammatory agents or selective modulators of immune response.
| |
| | |
| Structure of full-length Toxascaris leonina galectin with two carbohydrate-recognition domains.,Jeong MS, Hwang HG, Yu HS, Jang SB Acta Crystallogr D Biol Crystallogr. 2013 Feb;69(Pt 2):168-75. doi:, 10.1107/S0907444912045106. Epub 2013 Jan 16. PMID:23385453<ref>PMID:23385453</ref>
| |
| | |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| |
| </div>
| |
| <div class="pdbe-citations 4hl0" style="background-color:#fffaf0;"></div>
| |
|
| |
|
| ==See Also== | | ==See Also== |
| *[[Galectin|Galectin]] | | *[[Galectin 3D structures|Galectin 3D structures]] |
| == References ==
| |
| <references/>
| |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Parasitic roundworm]] | | [[Category: Toxascaris leonina]] |
| [[Category: Jeong, M S]] | | [[Category: Jeong MS]] |
| [[Category: A regulatory molecule]]
| |
| [[Category: Carbohydrate recognition domain]]
| |
| [[Category: Galectin]]
| |
| [[Category: Sugar binding protein]]
| |
| [[Category: The host immune system]]
| |