4dbh: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4dbh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DBH FirstGlance]. <br>
<table><tr><td colspan='2'>[[4dbh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum Corynebacterium glutamicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DBH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dbh OCA], [https://pdbe.org/4dbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dbh RCSB], [https://www.ebi.ac.uk/pdbsum/4dbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dbh ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dbh OCA], [https://pdbe.org/4dbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dbh RCSB], [https://www.ebi.ac.uk/pdbsum/4dbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dbh ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/Q8NQY2_CORGL Q8NQY2_CORGL]]
[https://www.uniprot.org/uniprot/Q8NQY2_CORGL Q8NQY2_CORGL]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cg1458 was recently characterized as a novel soluble oxaloacetate decarboxylase. However, sequence alignment identified that Cg1458 has no similarity with other oxaloacetate decarboxylases and instead belongs to the fumarylacetoacetate hydrolase (FAH) family. Differences in the function of Cg1458 and other FAH proteins may suggest a different catalytic mechanism. To help elucidate the catalytic mechanism of Cg1458, crystal structures of Cg1458 in both the open and closed conformations have been determined for the first time up to a resolution of 1.9 A and 2.0 A, respectively. Comparison of both structures and detailed biochemical studies confirmed the presence of a catalytic lid domain which is missing in the native enzyme structure. In this lid domain, a Glu-His dyad was found critical in mediating enzymatic catalysis. Based on structure modeling and comparison as well as large-scale sequence alignment studies, we further determined that the catalytic mechanism of Cg1458 is actually through a Glu-His-Water triad, and this catalytic triad is common among FAH family proteins that catalyze the cleavage of the C-C bond of the substrate. Two sequence motifs, HxxE and Hxx***xxE have been identified as the basis for this mechanism.
 
Crystal structures of Cg1458 reveal a catalytic lid domain and a common catalytic mechanism for FAH family.,Ran T, Gao Y, Marsh M, Zhu W, Wang M, Mao X, Xu L, Xu D, Wang W Biochem J. 2012 Oct 10. PMID:23046410<ref>PMID:23046410</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4dbh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 11:48, 20 March 2024

Crystal structure of Cg1458 with inhibitorCrystal structure of Cg1458 with inhibitor

Structural highlights

4dbh is a 2 chain structure with sequence from Corynebacterium glutamicum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.94Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8NQY2_CORGL

4dbh, resolution 1.94Å

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