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==Crystal Structure of Atlastin from Drosophila melanogaster==
==Crystal Structure of Atlastin from Drosophila melanogaster==
<StructureSection load='3x1d' size='340' side='right' caption='[[3x1d]], [[Resolution|resolution]] 2.87&Aring;' scene=''>
<StructureSection load='3x1d' size='340' side='right'caption='[[3x1d]], [[Resolution|resolution]] 2.87&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3x1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3X1D FirstGlance]. <br>
<table><tr><td colspan='2'>[[3x1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X1D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.87&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atl, CG6668 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3x1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x1d OCA], [http://pdbe.org/3x1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3x1d RCSB], [http://www.ebi.ac.uk/pdbsum/3x1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3x1d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x1d OCA], [https://pdbe.org/3x1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x1d RCSB], [https://www.ebi.ac.uk/pdbsum/3x1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x1d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/ATLAS_DROME ATLAS_DROME]] GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate microtubule polymerization and Golgi biogenesis. Required for dopaminergic neurons survival and the growth of muscles and synapses at neuromuscular junctions.<ref>PMID:19341724</ref> <ref>PMID:19633650</ref> 
[https://www.uniprot.org/uniprot/ATLAS_DROME ATLAS_DROME] GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate microtubule polymerization and Golgi biogenesis. Required for dopaminergic neurons survival and the growth of muscles and synapses at neuromuscular junctions.<ref>PMID:19341724</ref> <ref>PMID:19633650</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Formation of the endoplasmic reticulum (ER) network requires homotypic membrane fusion, which involves a class of atlastin (ATL) GTPases. Purified Drosophila ATL is capable of mediating vesicle fusion in vitro, but such activity has not been reported for any other ATLs. Here, we determined the preliminary crystal structure of the cytosolic segment of Drosophila ATL in a GDP-bound state. The structure reveals a GTPase domain dimer with the subsequent three-helix bundles associating with their own GTPase domains and pointing in opposite directions. This conformation is similar to that of human ATL1, to which GDP and high concentrations of inorganic phosphate, but not GDP only, were included. Drosophila ATL restored ER morphology defects in mammalian cells lacking ATLs, and measurements of nucleotide-dependent dimerization and GTPase activity were comparable for Drosophila ATL and human ATL1. However, purified and reconstituted human ATL1 exhibited no in vitro fusion activity. When the cytosolic segment of human ATL1 was connected to the transmembrane (TM) region and C-terminal tail (CT) of Drosophila ATL, the chimera still exhibited no fusion activity, though its GTPase activity was normal. These results suggest that GDP-bound ATLs may adopt multiple conformations and the in vitro fusion activity of ATL cannot be achieved by a simple collection of functional domains.
 
Comparison of human and Drosophila atlastin GTPases.,Wu F, Hu X, Bian X, Liu X, Hu J Protein Cell. 2015 Feb;6(2):139-46. doi: 10.1007/s13238-014-0118-0. Epub 2014 Nov, 20. PMID:25407413<ref>PMID:25407413</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3x1d" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Atlastin|Atlastin]]
*[[Atlastin 3D structures|Atlastin 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Drome]]
[[Category: Drosophila melanogaster]]
[[Category: Liu, X]]
[[Category: Large Structures]]
[[Category: Double helix]]
[[Category: Liu X]]
[[Category: Gtp binding]]
[[Category: Hydrolase]]
[[Category: Phosphorylation]]
[[Category: Rossmann fold]]

Latest revision as of 11:48, 20 March 2024

Crystal Structure of Atlastin from Drosophila melanogasterCrystal Structure of Atlastin from Drosophila melanogaster

Structural highlights

3x1d is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.87Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ATLAS_DROME GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate microtubule polymerization and Golgi biogenesis. Required for dopaminergic neurons survival and the growth of muscles and synapses at neuromuscular junctions.[1] [2]

See Also

References

  1. ↑ Lee M, Paik SK, Lee MJ, Kim YJ, Kim S, Nahm M, Oh SJ, Kim HM, Yim J, Lee CJ, Bae YC, Lee S. Drosophila Atlastin regulates the stability of muscle microtubules and is required for synapse development. Dev Biol. 2009 Jun 15;330(2):250-62. doi: 10.1016/j.ydbio.2009.03.019. Epub 2009 , Mar 31. PMID:19341724 doi:http://dx.doi.org/10.1016/j.ydbio.2009.03.019
  2. ↑ Orso G, Pendin D, Liu S, Tosetto J, Moss TJ, Faust JE, Micaroni M, Egorova A, Martinuzzi A, McNew JA, Daga A. Homotypic fusion of ER membranes requires the dynamin-like GTPase atlastin. Nature. 2009 Aug 20;460(7258):978-83. doi: 10.1038/nature08280. Epub 2009 Jul 26. PMID:19633650 doi:http://dx.doi.org/10.1038/nature08280

3x1d, resolution 2.87Å

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