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==Crystal structure of the computationally designed Pizza3 protein==
==Crystal structure of the computationally designed Pizza3 protein==
<StructureSection load='3ww8' size='340' side='right' caption='[[3ww8]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
<StructureSection load='3ww8' size='340' side='right'caption='[[3ww8]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3ww8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WW8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WW8 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3ww8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WW8 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.402&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ww7|3ww7]], [[3ww9|3ww9]], [[3wwa|3wwa]], [[3wwb|3wwb]], [[3wwf|3wwf]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ww8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ww8 OCA], [http://pdbe.org/3ww8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ww8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ww8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ww8 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ww8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ww8 OCA], [https://pdbe.org/3ww8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ww8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ww8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ww8 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The modular structure of many protein families, such as beta-propeller proteins, strongly implies that duplication played an important role in their evolution, leading to highly symmetrical intermediate forms. Previous attempts to create perfectly symmetrical propeller proteins have failed, however. We have therefore developed a new and rapid computational approach to design such proteins. As a test case, we have created a sixfold symmetrical beta-propeller protein and experimentally validated the structure using X-ray crystallography. Each blade consists of 42 residues. Proteins carrying 2-10 identical blades were also expressed and purified. Two or three tandem blades assemble to recreate the highly stable sixfold symmetrical architecture, consistent with the duplication and fusion theory. The other proteins produce different monodisperse complexes, up to 42 blades (180 kDa) in size, which self-assemble according to simple symmetry rules. Our procedure is suitable for creating nano-building blocks from different protein templates of desired symmetry.
Computational design of a self-assembling symmetrical beta-propeller protein.,Voet AR, Noguchi H, Addy C, Simoncini D, Terada D, Unzai S, Park SY, Zhang KY, Tame JR Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15102-7. doi:, 10.1073/pnas.1412768111. Epub 2014 Oct 6. PMID:25288768<ref>PMID:25288768</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ww8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Addy, C]]
[[Category: Large Structures]]
[[Category: Noguchi, H]]
[[Category: Addy C]]
[[Category: Park, S Y]]
[[Category: Noguchi H]]
[[Category: Simoncini, D]]
[[Category: Park SY]]
[[Category: Tame, J R.H]]
[[Category: Simoncini D]]
[[Category: Terada, D]]
[[Category: Tame JRH]]
[[Category: Unzai, S]]
[[Category: Terada D]]
[[Category: Voet, A R.D]]
[[Category: Unzai S]]
[[Category: Zhang, K Y.J]]
[[Category: Voet ARD]]
[[Category: Computational protein design]]
[[Category: Zhang KYJ]]
[[Category: De novo protein]]
[[Category: Self-assembly]]

Latest revision as of 11:47, 20 March 2024

Crystal structure of the computationally designed Pizza3 proteinCrystal structure of the computationally designed Pizza3 protein

Structural highlights

3ww8 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.402Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

3ww8, resolution 1.40Å

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