3m6n: Difference between revisions

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==Crystal structure of RpfF==
==Crystal structure of RpfF==
<StructureSection load='3m6n' size='340' side='right' caption='[[3m6n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='3m6n' size='340' side='right'caption='[[3m6n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3m6n]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Xance Xance]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M6N FirstGlance]. <br>
<table><tr><td colspan='2'>[[3m6n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M6N FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m6m|3m6m]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpfF, XCC1857 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=340 XANCE])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6n OCA], [https://pdbe.org/3m6n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m6n RCSB], [https://www.ebi.ac.uk/pdbsum/3m6n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m6n ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6n OCA], [http://pdbe.org/3m6n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3m6n RCSB], [http://www.ebi.ac.uk/pdbsum/3m6n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3m6n ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7CLS3_XANCP Q7CLS3_XANCP]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m6n ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m6n ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The diffusible signal factor (DSF)-dependent quorum sensing (QS) system adopts a novel protein-protein interaction mechanism to autoregulate the production of signal DSF. Here, we present the crystal structures of DSF synthase RpfF and its complex with the REC domain of sensor protein RpfC. RpfF is structurally similarity to the members of the crotonase superfamily and contains an N-terminal alpha/beta spiral core domain and a C-terminal alpha-helical region. Further structural and mutational analysis identified two catalytic glutamate residues, which is the conserved feature of the enoyl-CoA hydratases/dehydratases. A putative substrate-binding pocket was unveiled and the key roles of the residues implicated in substrate binding were verified by mutational analysis. The binding of the REC domain may lock RpfF in an inactive conformation by blocking the entrance of substrate binding pocket, thereby negatively regulating DSF production. These findings provide a structural model for the RpfC-RpfF interaction-mediated QS autoinduction mechanism.


Structural basis of the sensor-synthase interaction in autoinduction of the quorum sensing signal DSF biosynthesis.,Cheng Z, He YW, Lim SC, Qamra R, Walsh MA, Zhang LH, Song H Structure. 2010 Sep 8;18(9):1199-209. PMID:20826346<ref>PMID:20826346</ref>
==See Also==
 
*[[Enoyl-CoA hydratase 3D structures|Enoyl-CoA hydratase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3m6n" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Xance]]
[[Category: Large Structures]]
[[Category: Cheng, Z]]
[[Category: Xanthomonas campestris pv. campestris]]
[[Category: Lim, S C]]
[[Category: Cheng Z]]
[[Category: Qamra, R]]
[[Category: Lim SC]]
[[Category: Song, H]]
[[Category: Qamra R]]
[[Category: Enoyl-coa hydratase]]
[[Category: Song H]]
[[Category: Lyase]]
[[Category: Rpff]]

Latest revision as of 11:31, 20 March 2024

Crystal structure of RpfFCrystal structure of RpfF

Structural highlights

3m6n is a 3 chain structure with sequence from Xanthomonas campestris pv. campestris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7CLS3_XANCP

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

3m6n, resolution 1.80Å

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