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==Atomic model of the immature 50S subunit from Bacillus subtilis (state I-a)==
==Atomic model of the immature 50S subunit from Bacillus subtilis (state I-a)==
<StructureSection load='3j3v' size='340' side='right' caption='[[3j3v]], [[Resolution|resolution]] 13.30&Aring;' scene=''>
<SX load='3j3v' size='340' side='right' viewer='molstar' caption='[[3j3v]], [[Resolution|resolution]] 13.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3j3v]] is a 23 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J3V FirstGlance]. <br>
<table><tr><td colspan='2'>[[3j3v]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J3V FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j3w|3j3w]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j3v RCSB], [http://www.ebi.ac.uk/pdbsum/3j3v PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j3v OCA], [https://pdbe.org/3j3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j3v RCSB], [https://www.ebi.ac.uk/pdbsum/3j3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j3v ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/RL32_BACSU RL32_BACSU]
Ribosome assembly is a process fundamental for all cellular activities. The efficiency and accuracy of the subunit assembly are tightly regulated and closely monitored. In the present work, we characterized, both compositionally and structurally, a set of in vivo 50S subunit precursors (45S), isolated from a mutant bacterial strain. Our qualitative mass spectrometry data indicate that L28, L16, L33, L36 and L35 are dramatically underrepresented in the 45S particles. This protein spectrum shows interesting similarity to many qualitatively analyzed 50S precursors from different genetic background, indicating the presence of global rate-limiting steps in the late-stage assembly of 50S subunit. Our structural data reveal two major intermediate states for the 45S particles. Consistently, both states severally lack those proteins, but they also differ in the stability of the functional centers of the 50S subunit, demonstrating that they are translationally inactive. Detailed analysis indicates that the orientation of H38 accounts for the global conformational differences in these intermediate structures, and suggests that the reorientation of H38 to its native position is rate-limiting during the late-stage assembly. Especially, H38 plays an essential role in stabilizing the central protuberance, through the interaction with the 5S rRNA, and the correctly orientated H38 is likely a prerequisite for further maturation of the 50S subunit.
 
Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit.,Li N, Chen Y, Guo Q, Zhang Y, Yuan Y, Ma C, Deng H, Lei J, Gao N Nucleic Acids Res. 2013 May 21. PMID:23700310<ref>PMID:23700310</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Gao, N]]
[[Category: Large Structures]]
[[Category: Guo, Q]]
[[Category: Gao N]]
[[Category: Lei, J]]
[[Category: Guo Q]]
[[Category: Li, N]]
[[Category: Lei J]]
[[Category: Ma, C]]
[[Category: Li N]]
[[Category: Yuan, Y]]
[[Category: Ma C]]
[[Category: Zhang, Y]]
[[Category: Yuan Y]]
[[Category: Ribosome]]
[[Category: Zhang Y]]
[[Category: Ribosome assembly]]
[[Category: Ribosome biogenesis]]
[[Category: Rna folding]]
[[Category: Ylqf]]

Latest revision as of 11:28, 20 March 2024

Atomic model of the immature 50S subunit from Bacillus subtilis (state I-a)Atomic model of the immature 50S subunit from Bacillus subtilis (state I-a)

3j3v, resolution 13.30Å

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