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| ==Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM== | | ==Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM== |
| <StructureSection load='3j2f' size='340' side='right' caption='[[3j2f]], [[Resolution|resolution]] 17.60Å' scene=''> | | <SX load='3j2f' size='340' side='right' viewer='molstar' caption='[[3j2f]], [[Resolution|resolution]] 17.60Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3j2f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J2F FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3j2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J2F FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j28|3j28]], [[3j29|3j29]], [[3j2a|3j2a]], [[3j2b|3j2b]], [[3j2c|3j2c]], [[3j2d|3j2d]], [[3j2e|3j2e]], [[3j2g|3j2g]], [[3j2h|3j2h]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 17.6Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2f OCA], [http://pdbe.org/3j2f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j2f RCSB], [http://www.ebi.ac.uk/pdbsum/3j2f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j2f ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2f OCA], [https://pdbe.org/3j2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j2f RCSB], [https://www.ebi.ac.uk/pdbsum/3j2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j2f ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Ribosome biogenesis is a tightly regulated, multi-stepped process. The assembly of ribosomal subunits is a central step of the complex biogenesis process, involving nearly 30 protein factors in vivo in bacteria. Although the assembly process has been extensively studied in vitro for over 40 years, very limited information is known for the in vivo process and specific roles of assembly factors. Such an example is ribosome maturation factor M (RimM), a factor involved in the late-stage assembly of the 30S subunit. Here, we combined quantitative mass spectrometry and cryo-electron microscopy to characterize the in vivo 30S assembly intermediates isolated from mutant Escherichia coli strains with genes for assembly factors deleted. Our compositional and structural data show that the assembly of the 3'-domain of the 30S subunit is severely delayed in these intermediates, featured with highly underrepresented 3'-domain proteins and large conformational difference compared with the mature 30S subunit. Further analysis indicates that RimM functions not only to promote the assembly of a few 3'-domain proteins but also to stabilize the rRNA tertiary structure. More importantly, this study reveals intriguing similarities and dissimilarities between the in vitro and the in vivo assembly pathways, suggesting that they are in general similar but with subtle differences.
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| Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.,Guo Q, Goto S, Chen Y, Feng B, Xu Y, Muto A, Himeno H, Deng H, Lei J, Gao N Nucleic Acids Res. 2013 Jan 4. PMID:23293003<ref>PMID:23293003</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3j2f" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </SX> |
| [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| [[Category: Chen, Y]] | | [[Category: Large Structures]] |
| [[Category: Deng, H]] | | [[Category: Chen Y]] |
| [[Category: Gao, N]] | | [[Category: Deng H]] |
| [[Category: Goto, S]] | | [[Category: Gao N]] |
| [[Category: Guo, Q]] | | [[Category: Goto S]] |
| [[Category: Himeno, H]] | | [[Category: Guo Q]] |
| [[Category: Lei, J]] | | [[Category: Himeno H]] |
| [[Category: Muto, A]] | | [[Category: Lei J]] |
| [[Category: 30s subunit assembly]]
| | [[Category: Muto A]] |
| [[Category: Ribosome]]
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| [[Category: Ribosome biogenesis]]
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| [[Category: Rimm]]
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