UvrABC: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
Michal Harel (talk | contribs)
31,761 edits
No edit summary
 
(3 intermediate revisions by 2 users not shown)
Line 1: Line 1:
<StructureSection load='3pih' size='350' side='right' caption='UvrA complex with DNA, pyrophosphate and Zn+2 ion (grey) [[3pih]]' scene='' >
<StructureSection load='3pih' size='340' side='right' caption='UvrA complex with DNA, pyrophosphate and Zn+2 ion (grey) [[3pih]]' scene='53/536710/Cv/3' >
== Function ==
== Function ==
'''UvrABC''' endonuclease is an ''E. coli'' enzyme complex involved in DNA repair.  UvrABC removes 12 nucleotides around a DNA mutation replacing them with the correct one<ref>PMID:11004168</ref>.<br />
'''UvrABC''' endonuclease is an ''E. coli'' enzyme complex involved in DNA repair.  UvrABC removes 12 nucleotides around a DNA mutation replacing them with the correct one<ref>PMID:11004168</ref>.<br />
Line 11: Line 11:
== Structural highlights ==
== Structural highlights ==
UvrA contains several domains: <scene name='53/536710/Cv/2'>UvrB-binding, DNA-binding and two ATP-binding domains</scene><ref>PMID:21240268</ref>.
UvrA contains several domains: <scene name='53/536710/Cv/2'>UvrB-binding, DNA-binding and two ATP-binding domains</scene><ref>PMID:21240268</ref>.
</StructureSection>
 
==3D structures of UvrABC==
==3D structures of UvrABC==


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
[[UvrABC 3D structures]]
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*UvrA
 
**[[3pih]] – TmUvrA + DNA – ''Thermotoga maritima'' <br />
**[[6n9l]] - TmUvrA (mutant) + ADP<br />
**[[3zqj]] – UvrA  – ''Mycobacterium tuberculosis''<br />
**[[4dfc]] – EcUvrA + Transcription-repair-coupling factor – ''Escherichia coli'' <br />
**[[2r6f]] – BstUvrA – ''Bacillus stearothermophilus'' <br />
**[[3ux8]] – GeUvrA - ''Geobacillus''<br />
**[[2vf7]], [[2vf8]] – DrUvrA2 - ''Deinococcus radiodurans''<br />


*UvrB
**[[1d2m]], [[1c4o]] – UvrB – ''Thermus thermophilus'' <br />
**[[1d9x]] – BcUvrB – ''Bacillus caldotenax'' <br />
**[[1t5l]] – BcUvrB (mutant) <br />
**[[1qoj]] – EcUvrB C terminal <br />
**[[1e52]] – EcUvrB C terminal - NMR<br />
**[[1d9z]] – BcUvrB + ATP <br />
**[[2fdc]], [[6o8f]], [[6o8g]], [[6o8h]] – BcUvrB + DNA <br />
**[[6o8e]] – BcUvrB + DNA + ADP<br />
**[[2d7d]], [[2nmv]], [[3v4r]] – BsUvrB + DNA – ''Bacillus subtilis'' <br />
*UvrC
**[[1kft]], [[1qoj]] – EcUvrC C terminal <br />
**[[1e52]] – EcUvrC C terminal - NMR<br />
**[[2nrr]], [[2nrt]], [[2nrv]], [[2nrw]], [[2nrx]], [[2nrz]] – TmUvrC C terminal <br />
**[[1ycz]], [[1yd0]], [[1yd1]] – TmUvrC N terminal <br />
**[[1yd2]], [[1yd3]], [[1yd4]], [[1yd5]] – TmUvrC N terminal (mutant)<br />
**[[1yd6]] – BcUvrC N terminal <br />
**[[3c65]] – BstUvrC  <br />
*UvrD or DNA helicase II see [[Helicase]]
*UvrA-UvrB
**[[3fpn]] – BstUvrA + UvrB <br />
**[[3uwx]] – GeUvrA + UvrB <br />
}}
==References==
==References==
<references />
<references />
</StructureSection>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 10:52, 17 March 2024

Function

UvrABC endonuclease is an E. coli enzyme complex involved in DNA repair. UvrABC removes 12 nucleotides around a DNA mutation replacing them with the correct one[1].

  • UvrA is the protein which locates the DNA damage[2].
  • UvrB is involved in distinguishing damaged from undamaged DNA[3].
  • The C-terminal region of UvrC is involved in DNA binding and incisions at the 5'-side of a DNA damage during nucleotide excision repair[4].
  • UvrD is DNA helicase II. See Helicase.

For details on the UvrA-UvrB complex see UvrA-UvrB interaction domains.

Structural highlights

UvrA contains several domains: [5].

3D structures of UvrABC

UvrABC 3D structures

References

  1. ↑ Moolenaar GF, Moorman C, Goosen N. Role of the Escherichia coli nucleotide excision repair proteins in DNA replication. J Bacteriol. 2000 Oct;182(20):5706-14. PMID:11004168
  2. ↑ Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973
  3. ↑ Theis K, Skorvaga M, Machius M, Nakagawa N, Van Houten B, Kisker C. The nucleotide excision repair protein UvrB, a helicase-like enzyme with a catch. Mutat Res. 2000 Aug 30;460(3-4):277-300. PMID:10946234
  4. ↑ Moolenaar GF, Uiterkamp RS, Zwijnenburg DA, Goosen N. The C-terminal region of the Escherichia coli UvrC protein, which is homologous to the C-terminal region of the human ERCC1 protein, is involved in DNA binding and 5'-incision. Nucleic Acids Res. 1998 Jan 15;26(2):462-8. PMID:9421501
  5. ↑ Jaciuk M, Nowak E, Skowronek K, Tanska A, Nowotny M. Structure of UvrA nucleotide excision repair protein in complex with modified DNA. Nat Struct Mol Biol. 2011 Feb;18(2):191-7. Epub 2011 Jan 16. PMID:21240268 doi:10.1038/nsmb.1973

UvrA complex with DNA, pyrophosphate and Zn+2 ion (grey) 3pih

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=UvrABC&oldid=4101676"