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==Crystal structure of a MeaB- and MMAA-like GTPase from Mycobacterium tuberculosis bound to 2'-deoxyguanosine diphosphate==
==Crystal structure of a MeaB- and MMAA-like GTPase from Mycobacterium tuberculosis bound to 2'-deoxyguanosine diphosphate==
<StructureSection load='4gt1' size='340' side='right' caption='[[4gt1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='4gt1' size='340' side='right'caption='[[4gt1]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4gt1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GT1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4gt1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GT1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DGI:2-DEOXYGUANOSINE-5-DIPHOSPHATE'>DGI</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3md0|3md0]], [[3nxs|3nxs]], [[3tk1|3tk1]], [[3p32|3p32]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DGI:2-DEOXYGUANOSINE-5-DIPHOSPHATE'>DGI</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MT1543, MTCY277.18, Rv1496 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt1 OCA], [https://pdbe.org/4gt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gt1 RCSB], [https://www.ebi.ac.uk/pdbsum/4gt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gt1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gt1 OCA], [http://pdbe.org/4gt1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gt1 RCSB], [http://www.ebi.ac.uk/pdbsum/4gt1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gt1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/Y1496_MYCTU Y1496_MYCTU]] Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable).  
[https://www.uniprot.org/uniprot/Y1496_MYCTU Y1496_MYCTU] Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The methylmalonyl Co-A mutase-associated GTPase MeaB from Methylobacterium extorquens is involved in glyoxylate regulation and required for growth. In humans, mutations in the homolog methylmalonic aciduria associated protein (MMAA) cause methylmalonic aciduria, which is often fatal. The central role of MeaB from bacteria to humans suggests that MeaB is also important in other, pathogenic bacteria such as Mycobacterium tuberculosis. However, the identity of the mycobacterial MeaB homolog is presently unclear. Here, we identify the M. tuberculosis protein Rv1496 and its homologs in M. smegmatis and M. thermoresistibile as MeaB. The crystal structures of all three homologs are highly similar to MeaB and MMAA structures and reveal a characteristic three-domain homodimer with GDP bound in the G domain active site. A structure of Rv1496 obtained from a crystal grown in the presence of GTP exhibited electron density for GDP, suggesting GTPase activity. These structures identify the mycobacterial MeaB and provide a structural framework for therapeutic targeting of M. tuberculosis MeaB.
 
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.,Edwards TE, Baugh L, Bullen J, Baydo RO, Witte P, Thompkins K, Phan IQ, Abendroth J, Clifton MC, Sankaran B, Van Voorhis WC, Myler PJ, Staker BL, Grundner C, Lorimer DD J Struct Funct Genomics. 2015 Jun;16(2):91-9. doi: 10.1007/s10969-015-9197-2., Epub 2015 Apr 2. PMID:25832174<ref>PMID:25832174</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gt1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Structural genomic]]
[[Category: Large Structures]]
[[Category: Argk superfamily]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Atpase]]
[[Category: Chain swapped homodimer]]
[[Category: G-protein]]
[[Category: Gtpase]]
[[Category: Hydrolase]]
[[Category: Mycobacterium]]
[[Category: National institute of allergy and infectious disease]]
[[Category: Niaid]]
[[Category: Ras-like gtpase]]
[[Category: Ssgcid]]
[[Category: Substrate-specificity]]

Latest revision as of 18:55, 14 March 2024

Crystal structure of a MeaB- and MMAA-like GTPase from Mycobacterium tuberculosis bound to 2'-deoxyguanosine diphosphateCrystal structure of a MeaB- and MMAA-like GTPase from Mycobacterium tuberculosis bound to 2'-deoxyguanosine diphosphate

Structural highlights

4gt1 is a 1 chain structure with sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Y1496_MYCTU Probable GTPase. May also bind and hydrolyze ATP. May function as chaperone (Probable).

4gt1, resolution 2.00Å

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OCA
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