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| {{Large structure}}
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| ==Complex structure of Methane monooxygenase hydroxylase and regulatory subunit== | | ==Complex structure of Methane monooxygenase hydroxylase and regulatory subunit== |
| <StructureSection load='4gam' size='340' side='right' caption='[[4gam]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='4gam' size='340' side='right'caption='[[4gam]], [[Resolution|resolution]] 2.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4gam]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Metca Metca] and [http://en.wikipedia.org/wiki/Methylococcus_capsulatus Methylococcus capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GAM FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4gam]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylococcus_capsulatus_str._Bath Methylococcus capsulatus str. Bath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GAM FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.902Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1mmo|1mmo]], [[1ckv|1ckv]]</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mmoB, MCA1196 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243233 METCA])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gam OCA], [https://pdbe.org/4gam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gam RCSB], [https://www.ebi.ac.uk/pdbsum/4gam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gam ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methane_monooxygenase_(soluble) Methane monooxygenase (soluble)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.25 1.14.13.25] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gam OCA], [http://pdbe.org/4gam PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gam RCSB], [http://www.ebi.ac.uk/pdbsum/4gam PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gam ProSAT]</span></td></tr> | |
| </table> | | </table> |
| {{Large structure}}
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| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/MMOB_METCA MMOB_METCA]] The B protein acts as a regulator of electron flow through the soluble mmo complex, switching the enzyme from an oxidase to a hydroxylase in the presence of the substrate. [[http://www.uniprot.org/uniprot/MEMB_METCA MEMB_METCA]] Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. [[http://www.uniprot.org/uniprot/MEMG_METCA MEMG_METCA]] Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. [[http://www.uniprot.org/uniprot/MEMA_METCA MEMA_METCA]] Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. | | [https://www.uniprot.org/uniprot/MEMB_METCA MEMB_METCA] Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Methanotrophs consume methane as their major carbon source and have an essential role in the global carbon cycle by limiting escape of this greenhouse gas to the atmosphere. These bacteria oxidize methane to methanol by soluble and particulate methane monooxygenases (MMOs). Soluble MMO contains three protein components, a 251-kilodalton hydroxylase (MMOH), a 38.6-kilodalton reductase (MMOR), and a 15.9-kilodalton regulatory protein (MMOB), required to couple electron consumption with substrate hydroxylation at the catalytic diiron centre of MMOH. Until now, the role of MMOB has remained ambiguous owing to a lack of atomic-level information about the MMOH-MMOB (hereafter termed H-B) complex. Here we remedy this deficiency by providing a crystal structure of H-B, which reveals the manner by which MMOB controls the conformation of residues in MMOH crucial for substrate access to the active site. MMOB docks at the alpha(2)beta(2) interface of alpha(2)beta(2)gamma(2) MMOH, and triggers simultaneous conformational changes in the alpha-subunit that modulate oxygen and methane access as well as proton delivery to the diiron centre. Without such careful control by MMOB of these substrate routes to the diiron active site, the enzyme operates as an NADH oxidase rather than a monooxygenase. Biological catalysis involving small substrates is often accomplished in nature by large proteins and protein complexes. The structure presented in this work provides an elegant example of this principle.
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| Control of substrate access to the active site in methane monooxygenase.,Lee SJ, McCormick MS, Lippard SJ, Cho US Nature. 2013 Feb 21;494(7437):380-4. doi: 10.1038/nature11880. Epub 2013 Feb 10. PMID:23395959<ref>PMID:23395959</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4gam" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Methane monooxygenase|Methane monooxygenase]] | | *[[Methane monooxygenase 3D structures|Methane monooxygenase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Metca]] | | [[Category: Large Structures]] |
| [[Category: Methylococcus capsulatus]] | | [[Category: Methylococcus capsulatus str. Bath]] |
| [[Category: Cho, U S]] | | [[Category: Cho U-S]] |
| [[Category: Lee, S J]] | | [[Category: Lee SJ]] |
| [[Category: Lippard, S J]] | | [[Category: Lippard SJ]] |
| [[Category: Hydroxylase]]
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| [[Category: Methane monooxygenase]]
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| [[Category: Oxidoreductase]]
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| [[Category: Regulatory subunit b]]
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