4f1o: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
==Crystal Structure of the L1180T mutant Roco4 Kinase Domain from D. discoideum bound to AppCp==
==Crystal Structure of the L1180T mutant Roco4 Kinase Domain from D. discoideum bound to AppCp==
<StructureSection load='4f1o' size='340' side='right' caption='[[4f1o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='4f1o' size='340' side='right'caption='[[4f1o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4f1o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F1O FirstGlance]. <br>
<table><tr><td colspan='2'>[[4f1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F1O FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f0f|4f0f]], [[4f0g|4f0g]], [[4f1m|4f1m]], [[4f1t|4f1t]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DDB_G0288251, roco4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1o OCA], [https://pdbe.org/4f1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f1o RCSB], [https://www.ebi.ac.uk/pdbsum/4f1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f1o ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f1o RCSB], [http://www.ebi.ac.uk/pdbsum/4f1o PDBsum]</span></td></tr>
</table>
<table>
== Function ==
<div style="background-color:#fffaf0;">
[https://www.uniprot.org/uniprot/ROCO4_DICDI ROCO4_DICDI]
== Publication Abstract from PubMed ==
Mutations in human leucine-rich-repeat kinase 2 (LRRK2) have been found to be the most frequent cause of late-onset Parkinson disease. Here we show that Dictyostelium discoideum Roco4 is a suitable model to study the structural and biochemical characteristics of the LRRK2 kinase and can be used for optimization of current and identification of new LRRK2 inhibitors. We have solved the structure of Roco4 kinase wild-type, Parkinson disease-related mutants G1179S and L1180T (G2019S and I2020T in LRRK2) and the structure of Roco4 kinase in complex with the LRRK2 inhibitor H1152. Taken together, our data give important insight in the LRRK2 activation mechanism and, most importantly, explain the G2019S-related increase in LRRK2 kinase activity.
 
Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations.,Gilsbach BK, Ho FY, Vetter IR, van Haastert PJ, Wittinghofer A, Kortholt A Proc Natl Acad Sci U S A. 2012 Jun 11. PMID:22689969<ref>PMID:22689969</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Serine/threonine protein kinase|Serine/threonine protein kinase]]
*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
[[Category: Gilsbach, B K.]]
[[Category: Large Structures]]
[[Category: Kortholt, A.]]
[[Category: Gilsbach BK]]
[[Category: Vetter, I R.]]
[[Category: Kortholt A]]
[[Category: Wittinghofer, A.]]
[[Category: Vetter IR]]
[[Category: Atp-binding]]
[[Category: Wittinghofer A]]
[[Category: Kinase]]
[[Category: Lrrk2]]
[[Category: Nucleotide-binding]]
[[Category: Protein kinase]]
[[Category: Roco]]
[[Category: Serine/threonine-protein kinase]]
[[Category: Signaling protein]]
[[Category: Transferase]]

Latest revision as of 18:13, 14 March 2024

Crystal Structure of the L1180T mutant Roco4 Kinase Domain from D. discoideum bound to AppCpCrystal Structure of the L1180T mutant Roco4 Kinase Domain from D. discoideum bound to AppCp

Structural highlights

4f1o is a 1 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ROCO4_DICDI

See Also

4f1o, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=4f1o&oldid=4100525"