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==Crystal Structure of a Plectonemic RNA Supercoil==
==Crystal Structure of a Plectonemic RNA Supercoil==
<StructureSection load='4eya' size='340' side='right' caption='[[4eya]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='4eya' size='340' side='right'caption='[[4eya]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4eya]] is a 28 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EYA FirstGlance]. <br>
<table><tr><td colspan='2'>[[4eya]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EYA FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r2c|3r2c]], [[3r2d|3r2d]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_133, nusB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eya OCA], [https://pdbe.org/4eya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eya RCSB], [https://www.ebi.ac.uk/pdbsum/4eya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eya ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eya OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eya RCSB], [http://www.ebi.ac.uk/pdbsum/4eya PDBsum]</span></td></tr>
</table>
<table>
== Function ==
<div style="background-color:#fffaf0;">
[https://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE] Involved in the transcription termination process (By similarity).
== Publication Abstract from PubMed ==
Genome packaging is an essential housekeeping process in virtually all organisms for proper storage and maintenance of genetic information. Although the extent and mechanisms of packaging vary, the process involves the formation of nucleic-acid superstructures. Crystal structures of DNA coiled coils indicate that their geometries can vary according to sequence and/or the presence of stabilizers such as proteins or small molecules. However, such superstructures have not been revealed for RNA. Here we report the crystal structure of an RNA supercoil, which displays one level higher molecular organization than previously reported structures of DNA coiled coils. In the presence of an RNA-binding protein, two interlocking RNA coiled coils of double-stranded RNA, a 'coil of coiled coils', form a plectonemic supercoil. Molecular dynamics simulations suggest that protein-RNA interaction is required for the stability of the supercoiled RNA. This study provides structural insight into higher order packaging mechanisms of nucleic acids.
 
Crystal structure of a plectonemic RNA supercoil.,Stagno JR, Ma B, Li J, Altieri AS, Byrd RA, Ji X Nat Commun. 2012 Jun 12;3:901. doi: 10.1038/ncomms1903. PMID:22692544<ref>PMID:22692544</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus VF5]]
[[Category: Ji, X.]]
[[Category: Large Structures]]
[[Category: Stagno, J R.]]
[[Category: Synthetic construct]]
[[Category: Dsrna]]
[[Category: Ji X]]
[[Category: Nuse protein]]
[[Category: Stagno JR]]
[[Category: Ssrna]]
[[Category: Transcription]]
[[Category: Transcription elongation]]
[[Category: Transcription-rna complex]]

Latest revision as of 18:11, 14 March 2024

Crystal Structure of a Plectonemic RNA SupercoilCrystal Structure of a Plectonemic RNA Supercoil

Structural highlights

4eya is a 28 chain structure with sequence from Aquifex aeolicus VF5 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUSB_AQUAE Involved in the transcription termination process (By similarity).

4eya, resolution 3.20Å

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