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| ==Crystal structure of possible CutA1 divalent ion tolerance protein from Cryptosporidium parvum Iowa II== | | ==Crystal structure of possible CutA1 divalent ion tolerance protein from Cryptosporidium parvum Iowa II== |
| <StructureSection load='4e98' size='340' side='right' caption='[[4e98]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='4e98' size='340' side='right'caption='[[4e98]], [[Resolution|resolution]] 2.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4e98]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypi Crypi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E98 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E98 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4e98]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum_Iowa_II Cryptosporidium parvum Iowa II]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E98 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E98 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cgd6_2410 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=353152 CRYPI])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e98 OCA], [http://pdbe.org/4e98 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e98 RCSB], [http://www.ebi.ac.uk/pdbsum/4e98 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e98 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e98 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e98 OCA], [https://pdbe.org/4e98 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e98 RCSB], [https://www.ebi.ac.uk/pdbsum/4e98 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e98 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/CUTA_CRYPI CUTA_CRYPI] |
| Cryptosporidiosis is an infectious disease caused by protozoan parasites of the Cryptosporidium genus. Infection is associated with mild to severe diarrhea that usually resolves spontaneously in healthy human adults, but may lead to severe complications in young children and in immunocompromised patients. The genome of C. parvum contains a gene, CUTA_CRYPI, that may play a role in regulating the intracellular concentration of copper, which is a toxic element in excess. Here, the crystal structure of this CutA1 protein, Cp-CutA1, is reported at 2.0 A resolution. As observed for other CutA1 structures, the 117-residue protein is a trimer with a core ferrodoxin-like fold. Circular dichroism spectroscopy shows little, in any, unfolding of Cp-CutA1 up to 353 K. This robustness is corroborated by (1)H-(15)N HSQC spectra at 333 K, which are characteristic of a folded protein, suggesting that NMR spectroscopy may be a useful tool to further probe the function of the CutA1 proteins. While robust, Cp-CutA1 is not as stable as the homologous protein from a hyperthermophile, perhaps owing to a wide beta-bulge in beta2 that protrudes Pro48 and Ser49 outside the beta-sheet.
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| Structure of a CutA1 divalent-cation tolerance protein from Cryptosporidium parvum, the protozoal parasite responsible for cryptosporidiosis.,Buchko GW, Abendroth J, Clifton MC, Robinson H, Zhang Y, Hewitt SN, Staker BL, Edwards TE, Van Voorhis WC, Myler PJ Acta Crystallogr F Struct Biol Commun. 2015 May;71(Pt 5):522-30. doi:, 10.1107/S2053230X14028210. Epub 2015 Apr 18. PMID:25945704<ref>PMID:25945704</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4e98" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[CutA1|CutA1]] | | *[[CutA1 3D structures|CutA1 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Crypi]] | | [[Category: Cryptosporidium parvum Iowa II]] |
| [[Category: Buchko, G W]] | | [[Category: Large Structures]] |
| [[Category: Robinson, H]] | | [[Category: Buchko GW]] |
| [[Category: Structural genomic]] | | [[Category: Robinson H]] |
| [[Category: Signaling protein]]
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| [[Category: Ssgcid]]
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