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==Crystal Structure of PTE mutant K185R/I274N==
==Crystal Structure of PTE mutant K185R/I274N==
<StructureSection load='3ur5' size='340' side='right' caption='[[3ur5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='3ur5' size='340' side='right'caption='[[3ur5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3ur5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UR5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3ur5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UR5 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=DPF:DIETHYL+HYDROGEN+PHOSPHATE'>DPF</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=DPF:DIETHYL+HYDROGEN+PHOSPHATE'>DPF</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3upm|3upm]], [[3ur2|3ur2]], [[3ura|3ura]], [[3urb|3urb]], [[3urn|3urn]], [[3urq|3urq]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ur5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur5 OCA], [https://pdbe.org/3ur5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ur5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ur5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ur5 ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">opd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=293 Brevundimonas diminuta])</td></tr>
</table>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryldialkylphosphatase Aryldialkylphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.8.1 3.1.8.1] </span></td></tr>
== Function ==
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ur5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ur5 RCSB], [http://www.ebi.ac.uk/pdbsum/3ur5 PDBsum]</span></td></tr>
[https://www.uniprot.org/uniprot/OPD_BREDI OPD_BREDI] Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phosphotriesterase (PTE) from soil bacteria is known for its ability to catalyze the detoxification of organophosphate pesticides and chemical warfare agents. Most of the organophosphate chemical warfare agents are a mixture of two stereoisomers at the phosphorus center and the Sp-enantiomers are significantly more toxic than the Rp-enantiomers. In previous investigations PTE variants were created through the manipulation of the substrate binding pockets and these mutants were shown to have greater catalytic activities for the detoxification of the more toxic Sp-enantiomers of nerve agent analogs for GB, GD, GF, VX, and VR than the less toxic Rp- enantiomers. In this investigation alternate strategies were employed to discover additional PTE variants with significant improvements in catalytic activities relative to the wild type enzyme. Screening and selection techniques were utilized to isolate PTE variants from randomized libraries and site specific modifications. The catalytic activities of these newly identified PTE variants towards the Sp- enantiomers of chromophoric analogs of GB, GD, GF, VX, and VR have been improved up to 15,000 fold relative to the wild-type enzyme. The X-ray crystal structures of the best PTE variants were determined. Characterization of these mutants with the authentic G-type nerve agents has confirmed the expected improvements in catalytic activity against the most toxic enantiomers of GB, GD, and GF. The values of kcat/Km for the H257Y/L303T (YT) mutant for the hydrolysis of GB, GD, and GF were determined to be 2 x 106 M-1 s-1, 5 x 105 M-1 s-1, and 8 x 105 M-1 s-1, respectively. The YT mutant is the most proficient enzyme reported thus far for the detoxification of G-type nerve agents. These results support a combinatorial strategy of rational design and directed evolution as a powerful tool to discover more efficient enzymes for the detoxification of organophosphate nerve agents.
 
Enzymes for the Homeland Defense: Optimizing Phosphotriesterase for the Hydrolysis of Organophosphate Nerve Agents.,Tsai PC, Fox N, Bigley AN, Harvey SP, Barondeau DP, Raushel FM Biochemistry. 2012 Jul 18. PMID:22809162<ref>PMID:22809162</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Phosphotriesterase|Phosphotriesterase]]
*[[Phosphotriesterase 3D structures|Phosphotriesterase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aryldialkylphosphatase]]
[[Category: Brevundimonas diminuta]]
[[Category: Brevundimonas diminuta]]
[[Category: Barondeau, D P.]]
[[Category: Large Structures]]
[[Category: Fox, N G.]]
[[Category: Barondeau DP]]
[[Category: Li, Y.]]
[[Category: Fox NG]]
[[Category: Raushel, F M.]]
[[Category: Li Y]]
[[Category: Tsai, P.]]
[[Category: Raushel FM]]
[[Category: Hydrolase]]
[[Category: Tsai P]]
[[Category: Metalloenzyme]]
[[Category: Nerve agent]]
[[Category: Phosphotriesterase]]
[[Category: Tim barrel]]

Latest revision as of 17:06, 14 March 2024

Crystal Structure of PTE mutant K185R/I274NCrystal Structure of PTE mutant K185R/I274N

Structural highlights

3ur5 is a 2 chain structure with sequence from Brevundimonas diminuta. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OPD_BREDI Has an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates. All of the phosphate triesters found to be substrates are synthetic compounds. The identity of any naturally occurring substrate for the enzyme is unknown. Has no detectable activity with phosphate monoesters or diesters and no activity as an esterase or protease. It catalyzes the hydrolysis of the insecticide paraoxon at a rate approaching the diffusion limit and thus appears to be optimally evolved for utilizing this synthetic substrate.

See Also

3ur5, resolution 1.60Å

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