3uj3: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: '''Unreleased structure''' The entry 3uj3 is ON HOLD Authors: Ritacco, CJ, Wang, J, Kamtekar, S, Steitz, TA Description: Crystal Structure of the synaptic tetramer of the G-Segment Inv...
 
No edit summary
 
(8 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 3uj3 is ON HOLD
==Crystal Structure of the synaptic tetramer of the G-Segment Invertase (Gin)==
<StructureSection load='3uj3' size='340' side='right'caption='[[3uj3]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3uj3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3plo 3plo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UJ3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.51&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uj3 OCA], [https://pdbe.org/3uj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uj3 RCSB], [https://www.ebi.ac.uk/pdbsum/3uj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uj3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GIN_BPMU GIN_BPMU] Performs inversion of a viral 3 kp segment (G-segment) that encodes two alternate pairs of tail fiber proteins thereby modifying the host specificity of the virus. Binds as a dimer to the viral gix sites which are 34-bp palindromic sequences that flank the invertible G-segment. Catalyzes site-specific recombination in the presence of the host factor Fis. Gin dimers bound to each of the gix sites and host factor Fis bound to the enhancer come together to form the synaptic complex. Each Gin monomer introduces a nick and becomes covalently attached to the 5'-phosphate of the DNA, resulting in double-stranded staggered breaks at both recombination sites. A 180 degrees rotation of one of the two Gin dimers followed by religation of the DNA leads to the inversion of the G-segment (G+ or G- orientation).<ref>PMID:23275567</ref> <ref>PMID:2974801</ref> <ref>PMID:3159478</ref> <ref>PMID:6232613</ref>


Authors: Ritacco, CJ, Wang, J, Kamtekar, S, Steitz, TA
==See Also==
 
*[[Invertase|Invertase]]
Description: Crystal Structure of the synaptic tetramer of the G-Segment Invertase (Gin)
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia virus Mu]]
[[Category: Large Structures]]
[[Category: Kamtekar S]]
[[Category: Ritacco CJ]]
[[Category: Steitz TA]]
[[Category: Wang J]]

Latest revision as of 17:00, 14 March 2024

Crystal Structure of the synaptic tetramer of the G-Segment Invertase (Gin)Crystal Structure of the synaptic tetramer of the G-Segment Invertase (Gin)

Structural highlights

3uj3 is a 1 chain structure with sequence from Escherichia virus Mu. This structure supersedes the now removed PDB entry 3plo. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.51Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GIN_BPMU Performs inversion of a viral 3 kp segment (G-segment) that encodes two alternate pairs of tail fiber proteins thereby modifying the host specificity of the virus. Binds as a dimer to the viral gix sites which are 34-bp palindromic sequences that flank the invertible G-segment. Catalyzes site-specific recombination in the presence of the host factor Fis. Gin dimers bound to each of the gix sites and host factor Fis bound to the enhancer come together to form the synaptic complex. Each Gin monomer introduces a nick and becomes covalently attached to the 5'-phosphate of the DNA, resulting in double-stranded staggered breaks at both recombination sites. A 180 degrees rotation of one of the two Gin dimers followed by religation of the DNA leads to the inversion of the G-segment (G+ or G- orientation).[1] [2] [3] [4]

See Also

References

  1. ↑ Ritacco CJ, Kamtekar S, Wang J, Steitz TA. Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase. Nucleic Acids Res. 2013 Feb 1;41(4):2673-82. doi: 10.1093/nar/gks1303. Epub 2012 , Dec 28. PMID:23275567 doi:10.1093/nar/gks1303
  2. ↑ Klippel A, Cloppenborg K, Kahmann R. Isolation and characterization of unusual gin mutants. EMBO J. 1988 Dec 1;7(12):3983-9. doi: 10.1002/j.1460-2075.1988.tb03286.x. PMID:2974801 doi:http://dx.doi.org/10.1002/j.1460-2075.1988.tb03286.x
  3. ↑ Kahmann R, Rudt F, Koch C, Mertens G. G inversion in bacteriophage Mu DNA is stimulated by a site within the invertase gene and a host factor. Cell. 1985 Jul;41(3):771-80. doi: 10.1016/s0092-8674(85)80058-1. PMID:3159478 doi:http://dx.doi.org/10.1016/s0092-8674(85)80058-1
  4. ↑ Plasterk RH, Kanaar R, van de Putte P. A genetic switch in vitro: DNA inversion by Gin protein of phage Mu. Proc Natl Acad Sci U S A. 1984 May;81(9):2689-92. doi: 10.1073/pnas.81.9.2689. PMID:6232613 doi:http://dx.doi.org/10.1073/pnas.81.9.2689

3uj3, resolution 3.51Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3uj3&oldid=4098918"