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==Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine==
==Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine==
<StructureSection load='3tm4' size='340' side='right' caption='[[3tm4]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='3tm4' size='340' side='right'caption='[[3tm4]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3tm4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TM4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TM4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3tm4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TM4 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tlj|3tlj]], [[3tma|3tma]], [[3tm5|3tm5]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF1002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2261 Pyrococcus furiosus])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tm4 OCA], [https://pdbe.org/3tm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tm4 RCSB], [https://www.ebi.ac.uk/pdbsum/3tm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tm4 ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tm4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tm4 RCSB], [http://www.ebi.ac.uk/pdbsum/3tm4 PDBsum]</span></td></tr>
</table>
<table>
== Function ==
<div style="background-color:#fffaf0;">
[https://www.uniprot.org/uniprot/TRM14_PYRFU TRM14_PYRFU] S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Phe).<ref>PMID:22337946</ref> <ref>PMID:22362751</ref>  
== Publication Abstract from PubMed ==
Methyltransferases (MTases) form a major class of tRNA-modifying enzymes needed for the proper functioning of tRNA. Recently, RNA MTases from the TrmN/Trm14 family that are present in Archaea, Bacteria and Eukaryota have been shown to specifically modify tRNA(Phe) at guanosine 6 in the tRNA acceptor stem. Here, we report the first X-ray crystal structures of the tRNA m(2)G6 (N(2)-methylguanosine) MTase (TTC)TrmN from Thermus thermophilus and its ortholog (Pf)Trm14 from Pyrococcus furiosus. Structures of (Pf)Trm14 were solved in complex with the methyl donor S-adenosyl-l-methionine (SAM or AdoMet), as well as the reaction product S-adenosyl-homocysteine (SAH or AdoHcy) and the inhibitor sinefungin. (TTC)TrmN and (Pf)Trm14 consist of an N-terminal THUMP domain fused to a catalytic Rossmann-fold MTase (RFM) domain. These results represent the first crystallographic structure analysis of proteins containing both THUMP and RFM domain, and hence provide further insight in the contribution of the THUMP domain in tRNA recognition and catalysis. Electrostatics and conservation calculations suggest a main tRNA binding surface in a groove between the THUMP domain and the MTase domain. This is further supported by a docking model of TrmN in complex with tRNA(Phe) of T. thermophilus and via site-directed mutagenesis.
 
Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.,Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versees W Nucleic Acids Res. 2012 Feb 22. PMID:22362751<ref>PMID:22362751</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[TRNA methyltransferase|TRNA methyltransferase]]
*[[TRNA methyltransferase 3D structures|TRNA methyltransferase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pyrococcus furiosus]]
[[Category: Large Structures]]
[[Category: Bujnicki, J M.]]
[[Category: Pyrococcus furiosus DSM 3638]]
[[Category: Droogmans, L.]]
[[Category: Bujnicki JM]]
[[Category: Fislage, M.]]
[[Category: Droogmans L]]
[[Category: Roovers, M.]]
[[Category: Fislage M]]
[[Category: Tuszynska, I.]]
[[Category: Roovers M]]
[[Category: Versees, W.]]
[[Category: Tuszynska I]]
[[Category: Methyltransferase]]
[[Category: Versees W]]
[[Category: Rossmann fold]]
[[Category: Thump domain]]
[[Category: Transferase]]
[[Category: Trna methyltransferase]]

Latest revision as of 16:33, 14 March 2024

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionineCrystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine

Structural highlights

3tm4 is a 2 chain structure with sequence from Pyrococcus furiosus DSM 3638. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.95Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRM14_PYRFU S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Phe).[1] [2]

See Also

References

  1. ↑ Roovers M, Oudjama Y, Fislage M, Bujnicki JM, Versees W, Droogmans L. The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N(2)-methylguanosine at position 6 in tRNA. RNA. 2012 Apr;18(4):815-24. Epub 2012 Feb 15. PMID:22337946 doi:10.1261/rna.030411.111
  2. ↑ Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versees W. Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life. Nucleic Acids Res. 2012 Feb 22. PMID:22362751 doi:10.1093/nar/gks163

3tm4, resolution 1.95Å

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