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| ==Structure of a full-length bacterial LOV protein== | | ==Structure of a full-length bacterial LOV protein== |
| <StructureSection load='3sw1' size='340' side='right' caption='[[3sw1]], [[Resolution|resolution]] 2.63Å' scene=''> | | <StructureSection load='3sw1' size='340' side='right'caption='[[3sw1]], [[Resolution|resolution]] 2.63Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3sw1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SW1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SW1 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3sw1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_KT2440 Pseudomonas putida KT2440]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SW1 FirstGlance]. <br> |
| </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.63Å</td></tr> |
| <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PP4629 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sw1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sw1 RCSB], [http://www.ebi.ac.uk/pdbsum/3sw1 PDBsum]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sw1 OCA], [https://pdbe.org/3sw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sw1 RCSB], [https://www.ebi.ac.uk/pdbsum/3sw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sw1 ProSAT]</span></td></tr> |
| <table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q88E39_PSEPK Q88E39_PSEPK] |
| Blue-light photoreceptors containing light-oxygen-voltage (LOV) domains regulate a myriad of different physiological responses in both eukaryotes and prokaryotes. Their light sensitivity is intricately linked to the photochemistry of the non-covalently bound flavin mononucleotide (FMN) chromophore that forms a covalent adduct with a conserved cysteine residue in the LOV domain upon illumination with blue light. All LOV domains undergo the same primary photochemistry leading to adduct formation; however, considerable variation is found in the lifetime of the adduct state that varies from seconds to several hours. The molecular mechanism underlying this variation among the structurally conserved LOV protein family is not well understood. Here, we describe the structural characterization of PpSB1-LOV, a very slow cycling full-length LOV protein from the Gram-negative bacterium Pseudomonas putida KT2440. Its crystal structure reveals a novel dimer interface that is mediated by N- and C-terminal auxiliary structural elements and a unique cluster of four arginine residues coordinating with the FMN-phosphate moiety. Site-directed mutagenesis of two arginines (R61 and R66) in PpSB1-LOV resulted in acceleration of the dark recovery reaction approximately by a factor of 280. The presented structural and biochemical data suggest a direct link between structural features and the slow dark recovery observed for PpSB1-LOV. The overall structural arrangement of PpSB1-LOV, together with a complementary phylogenetic analysis, highlights a common ancestry of bacterial LOV photoreceptors and Per-ARNT-Sim chemosensors.
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| Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida.,Circolone F, Granzin J, Jentzsch K, Drepper T, Jaeger KE, Willbold D, Krauss U, Batra-Safferling R J Mol Biol. 2012 Apr 6;417(4):362-74. Epub 2012 Feb 7. PMID:22326872<ref>PMID:22326872</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Pseudomonas putida]] | | [[Category: Large Structures]] |
| [[Category: Batra-Safferling, R.]] | | [[Category: Phototropin]] |
| [[Category: Drepper, T.]] | | [[Category: Pseudomonas putida KT2440]] |
| [[Category: Granzin, J.]] | | [[Category: RCSB PDB Molecule of the Month]] |
| [[Category: Jaeger, K E.]] | | [[Category: Batra-Safferling R]] |
| [[Category: Krauss, U.]] | | [[Category: Drepper T]] |
| [[Category: Light-oxygen-voltage]] | | [[Category: Granzin J]] |
| [[Category: Lov]] | | [[Category: Jaeger K-E]] |
| [[Category: Pa]] | | [[Category: Krauss U]] |
| [[Category: Signaling protein]]
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