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[[Image:3sq3.png|left|200px]]


{{STRUCTURE_3sq3|  PDB=3sq3  |  SCENE=  }}
==Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H182A Mutant==
 
<StructureSection load='3sq3' size='340' side='right'caption='[[3sq3]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
===Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H182A Mutant===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3sq3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SQ3 FirstGlance]. <br>
{{ABSTRACT_PUBMED_22155078}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sq3 OCA], [https://pdbe.org/3sq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sq3 RCSB], [https://www.ebi.ac.uk/pdbsum/3sq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sq3 ProSAT]</span></td></tr>
==About this Structure==
</table>
[[3sq3]] is a 4 chain structure of [[Phosphodiesterase]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SQ3 OCA].  
== Function ==
[https://www.uniprot.org/uniprot/TYDP1_YEAST TYDP1_YEAST] DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Cleaves also 5' phosphotyrosyl adducts resulting from dead-end complexes between DNA and the active site tyrosine of topoisomerase II. Contributes to DNA repair after radiation damage. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. May have low 3'exonuclease activity and may be able to remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity).<ref>PMID:10521354</ref> <ref>PMID:16751265</ref>


==See Also==
==See Also==
*[[Phosphodiesterase|Phosphodiesterase]]
*[[Phosphodiesterase 3D structures|Phosphodiesterase 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:022155078</ref><references group="xtra"/>
__TOC__
[[Category: Saccharomyces cerevisiae]]
</StructureSection>
[[Category: Gajewski, S.]]
[[Category: Large Structures]]
[[Category: White, S W.]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Dna binding]]
[[Category: Gajewski S]]
[[Category: Hydrolase]]
[[Category: White SW]]
[[Category: Nuclear]]
[[Category: Phosphodiesterase]]

Latest revision as of 16:04, 14 March 2024

Crystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H182A MutantCrystal Structure Analysis of the Yeast Tyrosyl-DNA Phosphodiesterase H182A Mutant

Structural highlights

3sq3 is a 4 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TYDP1_YEAST DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Cleaves also 5' phosphotyrosyl adducts resulting from dead-end complexes between DNA and the active site tyrosine of topoisomerase II. Contributes to DNA repair after radiation damage. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. May have low 3'exonuclease activity and may be able to remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate (By similarity).[1] [2]

See Also

References

  1. ↑ Pouliot JJ, Yao KC, Robertson CA, Nash HA. Yeast gene for a Tyr-DNA phosphodiesterase that repairs topoisomerase I complexes. Science. 1999 Oct 15;286(5439):552-5. PMID:10521354
  2. ↑ Nitiss KC, Malik M, He X, White SW, Nitiss JL. Tyrosyl-DNA phosphodiesterase (Tdp1) participates in the repair of Top2-mediated DNA damage. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):8953-8. Epub 2006 Jun 2. PMID:16751265 doi:http://dx.doi.org/0603455103

3sq3, resolution 2.50Å

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