3rqw: Difference between revisions

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 ==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC==
-<StructureSection load='3rqw' size='340' side='right' caption='[[3rqw]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
+<StructureSection load='3rqw' size='340' side='right'caption='[[3rqw]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene><br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.913&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rqu|3rqu]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=204038 Dickeya dadantii])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [https://pdbe.org/3rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [https://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum]</span></td></tr>
+</table>
-<table>
+== Function ==
-<div style="background-color:#fffaf0;">
+[https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3]
-== Publication Abstract from PubMed ==
-ELIC, the pentameric ligand-gated ion channel from Erwinia chrysanthemi, is a prototype for Cys-loop receptors. Here we show that acetylcholine is a competitive antagonist for ELIC. We determine the acetylcholine-ELIC cocrystal structure to a 2.9-A resolution and find that acetylcholine binding to an aromatic cage at the subunit interface induces a significant contraction of loop C and other structural rearrangements in the extracellular domain. The side chain of the pore-lining residue F247 reorients and the pore size consequently enlarges, but the channel remains closed. We attribute the inability of acetylcholine to activate ELIC primarily to weak cation-pi and electrostatic interactions in the pocket, because an acetylcholine derivative with a simple quaternary-to-tertiary ammonium substitution activates the channel. This study presents a compelling case for understanding the structural underpinning of the functional relationship between agonism and competitive antagonism in the Cys-loop receptors, providing a new framework for developing novel therapeutic drugs.
-Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.,Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P Nat Commun. 2012 Mar 6;3:714. doi: 10.1038/ncomms1703. PMID:22395605<ref>PMID:22395605</ref>
-From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+==See Also==
-</div>
+*[[Ion channels 3D structures|Ion channels 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Dickeya dadantii]]
+[[Category: Dickeya dadantii 3937]]
-[[Category: Chen, Q.]]
+[[Category: Large Structures]]
-[[Category: Cohen, A.]]
+[[Category: Chen Q]]
-[[Category: Kong, X P.]]
+[[Category: Cohen A]]
-[[Category: Pan, J J.]]
+[[Category: Kong XP]]
-[[Category: Tang, P.]]
+[[Category: Pan JJ]]
-[[Category: Xu, Y.]]
+[[Category: Tang P]]
-[[Category: Yoshida, K.]]
+[[Category: Xu Y]]
-[[Category: Ion channel]]
+[[Category: Yoshida K]]
-[[Category: Membrane]]
-[[Category: Transport protein]]