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| ==Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA== | | ==Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA== |
| <StructureSection load='3r2c' size='340' side='right' caption='[[3r2c]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='3r2c' size='340' side='right'caption='[[3r2c]], [[Resolution|resolution]] 1.90Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3r2c]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2C FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3r2c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2C FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.902Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r2d|3r2d]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_133, nusB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]), aq_008, NUSE, rpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2c OCA], [https://pdbe.org/3r2c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2c RCSB], [https://www.ebi.ac.uk/pdbsum/3r2c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2c ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r2c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r2c RCSB], [http://www.ebi.ac.uk/pdbsum/3r2c PDBsum]</span></td></tr> | |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE]] Involved in the transcription termination process (By similarity). [[http://www.uniprot.org/uniprot/RS10_AQUAE RS10_AQUAE]] Involved in the binding of tRNA to the ribosomes.[HAMAP-Rule:MF_00508] | | [https://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE] Involved in the transcription termination process (By similarity). |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Processive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8 nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural observations and establish the relative significance of key protein-protein and protein-RNA interactions. Further crystallographic investigation of a NusB-NusE-dsRNA complex reveals a heretofore unobserved dsRNA binding site contiguous with the BoxA binding site. We propose that the observed dsRNA represents BoxB RNA, as both single-stranded BoxA and double-stranded BoxB components are present in the classical lambda antitermination site. Combining these data with known interactions amongst antitermination factors suggests a specific model for the assembly of the complete antitermination complex.
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| Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.,Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X Nucleic Acids Res. 2011 Jun 7. PMID:21652641<ref>PMID:21652641</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| ==See Also== | | ==See Also== |
| *[[Ribosomal protein S10|Ribosomal protein S10]] | | *[[Ribosomal protein S10|Ribosomal protein S10]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Aquifex aeolicus]] | | [[Category: Aquifex aeolicus]] |
| [[Category: Ji, X]] | | [[Category: Large Structures]] |
| [[Category: Stagno, J R]] | | [[Category: Ji X]] |
| [[Category: Cross species nusb-nuse-rna interaction]] | | [[Category: Stagno JR]] |
| [[Category: Gene regulation]]
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| [[Category: Protein-rna interaction]]
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| [[Category: Transcription elongation]]
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| [[Category: Transcription-rna complex]]
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