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| <StructureSection load='3qo7' size='340' side='right'caption='[[3qo7]], [[Resolution|resolution]] 2.55Å' scene=''> | | <StructureSection load='3qo7' size='340' side='right'caption='[[3qo7]], [[Resolution|resolution]] 2.55Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3qo7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11006_[[monilia_stellatoidea]] Atcc 11006 [[monilia stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QO7 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3qo7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QO7 FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qne|3qne]], [[3qo5|3qo5]], [[3qo8|3qo8]]</div></td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SES1, CaO19.7901 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Monilia stellatoidea]]])</td></tr> | |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Serine--tRNA_ligase Serine--tRNA ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.11 6.1.1.11] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qo7 OCA], [https://pdbe.org/3qo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qo7 RCSB], [https://www.ebi.ac.uk/pdbsum/3qo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qo7 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qo7 OCA], [https://pdbe.org/3qo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qo7 RCSB], [https://www.ebi.ac.uk/pdbsum/3qo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qo7 ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[https://www.uniprot.org/uniprot/SYSC_CANAL SYSC_CANAL]] Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
| | [https://www.uniprot.org/uniprot/SYSC_CANAL SYSC_CANAL] Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| In a restricted group of opportunistic fungal pathogens the universal leucine CUG codon is translated both as serine (97%) and leucine (3%), challenging the concept that translational ambiguity has a negative impact in living organisms. To elucidate the molecular mechanisms underlying the in vivo tolerance to a nonconserved genetic code alteration, we have undertaken an extensive structural analysis of proteins containing CUG-encoded residues and solved the crystal structures of the two natural isoforms of Candida albicans seryl-tRNA synthetase. We show that codon reassignment resulted in a nonrandom genome-wide CUG redistribution tailored to minimize protein misfolding events induced by the large-scale leucine-to-serine replacement within the CTG clade. Leucine or serine incorporation at the CUG position in C. albicans seryl-tRNA synthetase induces only local structural changes and, although both isoforms display tRNA serylation activity, the leucine-containing isoform is more active. Similarly, codon ambiguity is predicted to shape the function of C. albicans proteins containing CUG-encoded residues in functionally relevant positions, some of which have a key role in signaling cascades associated with morphological changes and pathogenesis. This study provides a first detailed analysis on natural reassignment of codon identity, unveiling a highly dynamic evolutionary pattern of thousands of fungal CUG codons to confer an optimized balance between protein structural robustness and functional plasticity.
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| Unveiling the structural basis for translational ambiguity tolerance in a human fungal pathogen.,Rocha R, Pereira PJ, Santos MA, Macedo-Ribeiro S Proc Natl Acad Sci U S A. 2011 Aug 8. PMID:21825144<ref>PMID:21825144</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3qo7" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] | | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| | [[Category: Candida albicans]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Serine--tRNA ligase]]
| | [[Category: Macedo-Ribeiro S]] |
| [[Category: Macedo-Ribeiro, S]] | | [[Category: Pereira PJB]] |
| [[Category: Pereira, P J.B]] | | [[Category: Rocha R]] |
| [[Category: Rocha, R]] | | [[Category: Santos MA]] |
| [[Category: Santos, M A]] | |
| [[Category: Amino acid biosynthesis]]
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| [[Category: Aminoacyl-trna synthetase]]
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| [[Category: Class-ii aminoacyl-trna synthetase family]]
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| [[Category: Codon ambiguity]]
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| [[Category: Ctg-clade]]
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| [[Category: Ligase]]
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| [[Category: Pathogen]]
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| [[Category: Serine]]
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| [[Category: Trna]]
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| [[Category: Type-1 seryl-trna synthetase subfamily]]
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