1d7k: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /> <applet load="1d7k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d7k, resolution 2.1Å" /> '''CRYSTAL STRUCTURE OF...
 
No edit summary
 
(17 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1d7k.gif|left|200px]]<br />
<applet load="1d7k" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d7k, resolution 2.1&Aring;" />
'''CRYSTAL STRUCTURE OF HUMAN ORNITHINE DECARBOXYLASE AT 2.1 ANGSTROMS RESOLUTION'''<br />


==Overview==
==CRYSTAL STRUCTURE OF HUMAN ORNITHINE DECARBOXYLASE AT 2.1 ANGSTROMS RESOLUTION==
The polyamines spermidine and spermine are ubiquitous and required for, cell growth and differentiation in eukaryotes. Ornithine decarboxylase, (ODC, EC 4.1.1.17) performs the first step in polyamine biosynthesis, the, decarboxylation of ornithine to putrescine. Elevated polyamine levels can, lead to down-regulation of ODC activity by enhancing the translation of, antizyme mRNA, resulting in subsequent binding of antizyme to ODC monomers, which targets ODC for proteolysis by the 26S proteasome.The crystal, structure of ornithine decarboxylase from human liver has been determined, to 2.1 A resolution by molecular replacement using truncated mouse ODC, (Delta425-461) as the search model and refined to a crystallographic, R-factor of 21.2% and an R-free value of 28.8%. The human ODC model, includes several regions that are disordered in the mouse ODC crystal, structure, including one of two C-terminal basal degradation elements that, have been demonstrated to independently collaborate with antizyme binding, to target ODC for degradation by the 26S proteasome.The crystal structure, of human ODC suggests that the C terminus, which contains basal, degradation elements necessary for antizyme-induced proteolysis, is not, buried by the structural core of homodimeric ODC as previously proposed., Analysis of the solvent-accessible surface area, surface electrostatic, potential, and the conservation of primary sequence between human ODC and, Trypanosoma brucei ODC provides clues to the identity of potential, protein-binding-determinants in the putative antizyme binding element in, human ODC.
<StructureSection load='1d7k' size='340' side='right'caption='[[1d7k]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1d7k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D7K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7k OCA], [https://pdbe.org/1d7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d7k RCSB], [https://www.ebi.ac.uk/pdbsum/1d7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d7k ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DCOR_HUMAN DCOR_HUMAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/1d7k_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d7k ConSurf].
<div style="clear:both"></div>


==Disease==
==See Also==
Known disease associated with this structure: Colonic adenoma recurrence, reduced risk of OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=165640 165640]]
*[[Ornithine decarboxylase|Ornithine decarboxylase]]
 
__TOC__
==About this Structure==
</StructureSection>
1D7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ornithine_decarboxylase Ornithine decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.17 4.1.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D7K OCA].
 
==Reference==
Crystal structure of human ornithine decarboxylase at 2.1 A resolution: structural insights to antizyme binding., Almrud JJ, Oliveira MA, Kern AD, Grishin NV, Phillips MA, Hackert ML, J Mol Biol. 2000 Jan 7;295(1):7-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10623504 10623504]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Ornithine decarboxylase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Almrud JJ]]
[[Category: Almrud, J.J.]]
[[Category: Grishin NV]]
[[Category: Grishin, N.V.]]
[[Category: Hackert ML]]
[[Category: Hackert, M.L.]]
[[Category: Kern AD]]
[[Category: Kern, A.D.]]
[[Category: Oliveira MA]]
[[Category: Oliveira, M.A.]]
[[Category: Phillips MA]]
[[Category: Phillips, M.A.]]
[[Category: alpha-beta barrel]]
[[Category: decarboxylation]]
[[Category: ornithine]]
[[Category: pyridoxal 5'-phosphate]]
[[Category: sheet-domain]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:30:01 2007''

Latest revision as of 18:47, 13 March 2024

CRYSTAL STRUCTURE OF HUMAN ORNITHINE DECARBOXYLASE AT 2.1 ANGSTROMS RESOLUTIONCRYSTAL STRUCTURE OF HUMAN ORNITHINE DECARBOXYLASE AT 2.1 ANGSTROMS RESOLUTION

Structural highlights

1d7k is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DCOR_HUMAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1d7k, resolution 2.10Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1d7k&oldid=4095168"