1crx: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1crx.gif|left|200px]]<br /><applet load="1crx" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1crx, resolution 2.4&Aring;" />
-'''CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I'''<br />
-==Overview==
+==CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I==
-During site-specific DNA recombination, which brings about genetic, rearrangement in processes such as viral integration and excision and, chromosomal segregation, recombinase enzymes recognize specific DNA, sequences and catalyse the reciprocal exchange of DNA strands between, these sites. The bacteriophage recombinase Cre catalyses site-specific, recombination between two 34-base-pair loxP sites. The crystal structure, at 2.4 A resolution of Cre bound to a loxP substrate reveals an, intermediate in the recombination reaction, in which a Cre molecule has, cleaved the substrate to form a covalent 3'-phosphotyrosine linkage with, the DNA. Four recombinases and two loxP sites form a synapsed structure in, which the DNA resembles models of four-way Holliday-Junction, intermediates. The Cre-loxP complex challenges models of site-specific, recombination that require large changes in quaternary structure. Subtle, allosteric changes at the carboxy termini of the Cre subunits may instead, coordinate the cleavage and strand-exchange reactions.
+<StructureSection load='1crx' size='340' side='right'caption='[[1crx]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1crx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P1 Escherichia virus P1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CRX FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1crx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crx OCA], [https://pdbe.org/1crx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1crx RCSB], [https://www.ebi.ac.uk/pdbsum/1crx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1crx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RECR_BPP1 RECR_BPP1] Catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1crx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1crx ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-CRX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CRX OCA].
+*[[Resolvase 3D structures|Resolvase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse., Guo F, Gopaul DN, van Duyne GD, Nature. 1997 Sep 4;389(6646):40-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9288963 9288963]
+[[Category: Escherichia virus P1]]
-[[Category: Enterobacteria phage p21]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Gopaul DN]]
-[[Category: Duyne, G.D.Van.]]
+[[Category: Guo F]]
-[[Category: Gopaul, D.N.]]
+[[Category: Van Duyne GD]]
-[[Category: Guo, F.]]
-[[Category: PO4]]
-[[Category: complex (recombinase/dna)]]
-[[Category: protein:dna complex]]
-[[Category: reaction intermediate]]
-[[Category: site-specific recombinase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:42:47 2007''