1cdg: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1cdg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cdg, resolution 2.0Å" /> '''NUCLEOTIDE SEQUENCE A...
 
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1cdg.gif|left|200px]]<br /><applet load="1cdg" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cdg, resolution 2.0&Aring;" />
'''NUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 IN A MALTOSE-DEPENDENT CRYSTAL FORM'''<br />


==Overview==
==NUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 IN A MALTOSE-DEPENDENT CRYSTAL FORM==
The cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) gene from, Bacillus circulans strain 251 was cloned and sequenced. It was found to, code for a mature protein of 686 amino acid residues, showing 75% identity, to the CGTase from B. circulans strain 8. The X-ray structure of the, CGTase was elucidated in a maltodextrin-dependent crystal form and refined, against X-ray diffraction data to 2.0 A resolution. The structure of the, enzyme is nearly identical to the CGTase from B. circulans strain 8. Three, maltose binding sites are observed at the protein surface, two in domain E, and one in domain C. The maltose-dependence of CGTase crystallization can, be ascribed to the proximity of two of the maltose binding sites to, intermolecular crystal contacts. The maltose molecules bound in the E, domain interact with several residues implicated in a raw starch binding, motif conserved among a diverse group of starch converting enzymes.
<StructureSection load='1cdg' size='340' side='right'caption='[[1cdg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cdg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CDG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CDG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cdg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cdg OCA], [https://pdbe.org/1cdg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cdg RCSB], [https://www.ebi.ac.uk/pdbsum/1cdg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cdg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CDGT2_NIACI CDGT2_NIACI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/1cdg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cdg ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CDG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with MAL and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CDG OCA].
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form., Lawson CL, van Montfort R, Strokopytov B, Rozeboom HJ, Kalk KH, de Vries GE, Penninga D, Dijkhuizen L, Dijkstra BW, J Mol Biol. 1994 Feb 18;236(2):590-600. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8107143 8107143]
[[Category: Large Structures]]
[[Category: Bacillus circulans]]
[[Category: Niallia circulans]]
[[Category: Cyclomaltodextrin glucanotransferase]]
[[Category: Dijkstra BW]]
[[Category: Single protein]]
[[Category: Kalk KH]]
[[Category: Dijkstra, B.W.]]
[[Category: Lawson CL]]
[[Category: Kalk, K.H.]]
[[Category: Rozeboom HJ]]
[[Category: Lawson, C.L.]]
[[Category: Strokopytov BV]]
[[Category: Montfort, R.Van.]]
[[Category: Van Montfort R]]
[[Category: Rozeboom, H.J.]]
[[Category: Strokopytov, B.V.]]
[[Category: CA]]
[[Category: MAL]]
[[Category: transferase(glucanotransferase)]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:22:01 2007''

Latest revision as of 18:39, 13 March 2024

NUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 IN A MALTOSE-DEPENDENT CRYSTAL FORMNUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 IN A MALTOSE-DEPENDENT CRYSTAL FORM

Structural highlights

1cdg is a 1 chain structure with sequence from Niallia circulans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CDGT2_NIACI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1cdg, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1cdg&oldid=4094577"