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| ==DETERMINATION OF THE NMR SOLUTION STRUCTURE OF AN ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX== | | ==DETERMINATION OF THE NMR SOLUTION STRUCTURE OF AN ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX== |
| <StructureSection load='1ahd' size='340' side='right' caption='[[1ahd]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''> | | <StructureSection load='1ahd' size='340' side='right'caption='[[1ahd]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[1ahd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosu Drosu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AHD FirstGlance]. <br> | | <table><tr><td colspan='2'>[[1ahd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_subobscura Drosophila subobscura]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AHD FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Antp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7241 DROSU])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ahd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahd OCA], [http://pdbe.org/1ahd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ahd RCSB], [http://www.ebi.ac.uk/pdbsum/1ahd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahd ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ahd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ahd OCA], [https://pdbe.org/1ahd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ahd RCSB], [https://www.ebi.ac.uk/pdbsum/1ahd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ahd ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/ANTP_DROSU ANTP_DROSU]] Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis (By similarity).[UniProtKB:P02833] | | [https://www.uniprot.org/uniprot/ANTP_DROSU ANTP_DROSU] Sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. Provides cells with specific positional identities on the anterior-posterior axis (By similarity).[UniProtKB:P02833] |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahd ConSurf]. | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ahd ConSurf]. |
| <div style="clear:both"></div> | | <div style="clear:both"></div> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The nuclear magnetic resonance (NMR) solution structure of a complex formed by the mutant Antennapedia homeodomain with Cys39 replaced by Ser, Antp(C39S), and a 14 base-pair DNA duplex containing the BS2 operator sequence was determined using uniform 13C and 15N-labeling of the protein. Two-dimensional nuclear Overhauser enhancement spectroscopy ([1H,1H]NOESY) with 15N(omega 2)-half-filter and 13C(omega 1, omega 2)-double-half-filter, and three-dimensional heteronuclear-correlated [1H,1H]NOESY yielded a total of 855 intramolecular NOE upper distance constraints in the homeodomain, 151 upper distance constraints within the DNA duplex, and 39 intermolecular protein-DNA upper distance constraints. These data were used as the input for the structure calculation with simulated annealing followed by molecular dynamics in a water bath and energy refinement. A group of 16 conformers was thus generated which represent the solution structure of the Antp(C39S) homeodomain-DNA complex. The new structure determination confirms the salient features reported previously from a preliminary investigation of the same complex, in particular the location of the recognition helix in the major groove with the turn of the helix-turn-helix motif outside the contact area with the DNA, and the N-terminal arm of the homeodomain contacting the minor groove of the DNA. In addition, distinct amino acid side-chain-DNA contacts could be identified, and evidence was found that the invariant residue Asn51 (and possibly also Gln50) is in a slow dynamic equilibrium between two or several different DNA contact sites. The molecular dynamics calculations in a water bath yielded structures with hydration water molecules in the protein-DNA interface, which coincides with direct NMR observations of hydration waters. In the Appendix the experimental data obtained with the Antp(C39S) homeodomain-DNA complex and the techniques used for the structure calculation are evaluated using a simulated input data set derived from the X-ray crystal structure of a DNA complex with a homologous homeodomain. This study indicates that a nearly complete set of NOE upper distance constraints for the Antp(C39S) homeodomain and the protein-DNA interface was presently obtained. It further shows that the structure calculation used here yields a precise reproduction of the crystal structure from the simulated input data, and also results in hydration of the protein-DNA interface in the recalculated complex.
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| Determination of the nuclear magnetic resonance solution structure of an Antennapedia homeodomain-DNA complex.,Billeter M, Qian YQ, Otting G, Muller M, Gehring W, Wuthrich K J Mol Biol. 1993 Dec 20;234(4):1084-93. PMID:7903398<ref>PMID:7903398</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 1ahd" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Drosu]] | | [[Category: Drosophila subobscura]] |
| [[Category: Billeter, M]] | | [[Category: Large Structures]] |
| [[Category: Gehring, W J]] | | [[Category: Billeter M]] |
| [[Category: Muller, M]] | | [[Category: Gehring WJ]] |
| [[Category: Otting, G]] | | [[Category: Muller M]] |
| [[Category: Qian, Y Q]] | | [[Category: Otting G]] |
| [[Category: Wuthrich, K]] | | [[Category: Qian YQ]] |
| [[Category: Dna binding protein-dna complex]]
| | [[Category: Wuthrich K]] |
| [[Category: Dna binding protein/dna]]
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