6dnf: Difference between revisions

<StructureSection load='6dnf' size='340' side='right' caption='[[6dnf]], [[Resolution|resolution]] 3.20&Aring;' scene=''>

<table><tr><td colspan='2'>[[6dnf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DNF FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DGG:1-[GLYCEROLYLPHOSPHONYL]-2-[8-(2-HEXYL-CYCLOPROPYL)-OCTANAL-1-YL]-3-[HEXADECANAL-1-YL]-GLYCEROL'>DGG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dnf OCA], [http://pdbe.org/6dnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dnf RCSB], [http://www.ebi.ac.uk/pdbsum/6dnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dnf ProSAT]</span></td></tr>

== Publication Abstract from PubMed ==

The mitochondrial calcium uniporter (MCU) is a highly selective calcium channel and a major route of calcium entry into mitochondria. How the channel catalyses ion permeation and achieves ion selectivity are not well understood, partly because MCU is thought to have a distinct architecture in comparison to other cellular channels. Here we report cryo-electron microscopy reconstructions of MCU channels from zebrafish and Cyphellophora europaea at 8.5 A and 3.2 A resolutions, respectively. In contrast to a previous report of pentameric stoichiometry for MCU, both channels are tetramers. The atomic model of C. europaea MCU shows that a conserved WDXXEP signature sequence forms the selectivity filter, in which calcium ions are arranged in single file. Coiled-coil legs connect the pore to N-terminal domains in the mitochondrial matrix. In C. europaea MCU, the N-terminal domains assemble as a dimer of dimers; in zebrafish MCU, they form an asymmetric crescent. The structures define principles that underlie ion permeation and calcium selectivity in this unusual channel.

 

<div class="pdbe-citations 6dnf" style="background-color:#fffaf0;"></div>

== References ==

</StructureSection>

[[Category: Baradaran, R]]

[[Category: Long, S B]]

[[Category: Wang, C]]

[[Category: Calcium]]

[[Category: Eukaryotic]]

[[Category: Mitochondria]]