6clf: Difference between revisions

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 ==1.15 A MicroED structure of GSNQNNF at 1.9 e- / A^2==
-<StructureSection load='6clf' size='340' side='right' caption='[[6clf]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
+<StructureSection load='6clf' size='340' side='right'caption='[[6clf]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6clf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CLF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6clf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CLF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6clf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6clf OCA], [http://pdbe.org/6clf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6clf RCSB], [http://www.ebi.ac.uk/pdbsum/6clf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6clf ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6clf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6clf OCA], [https://pdbe.org/6clf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6clf RCSB], [https://www.ebi.ac.uk/pdbsum/6clf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6clf ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.
-Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.,Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T Structure. 2018 May 1;26(5):759-766.e4. doi: 10.1016/j.str.2018.03.021. Epub 2018, Apr 26. PMID:29706530<ref>PMID:29706530</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 6clf" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Gallagher-Jones, M]]
+[[Category: Large Structures]]
-[[Category: Glynn, C]]
+[[Category: Synthetic construct]]
-[[Category: Gonen, T]]
+[[Category: Gallagher-Jones M]]
-[[Category: Hattne, J]]
+[[Category: Glynn C]]
-[[Category: Martynowycz, M W]]
+[[Category: Gonen T]]
-[[Category: Rodriguez, J A]]
+[[Category: Hattne J]]
-[[Category: Shi, D]]
+[[Category: Martynowycz MW]]
-[[Category: Zee, C T]]
+[[Category: Rodriguez JA]]
-[[Category: Amyloid fibril]]
+[[Category: Shi D]]
-[[Category: Prion]]
+[[Category: Zee C-T]]
-[[Category: Protein fibril]]
-[[Category: Zinc binding]]