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{{Large structure}}
 
==Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex==
==Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex==
<StructureSection load='5uz9' size='340' side='right' caption='[[5uz9]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
<SX load='5uz9' size='340' side='right' viewer='molstar' caption='[[5uz9]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5uz9]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpd31 Bpd31], [http://en.wikipedia.org/wiki/Pseab Pseab] and [http://en.wikipedia.org/wiki/Pseudomonas_phage_jbd30 Pseudomonas phage jbd30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZ9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5uz9]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14], [https://en.wikipedia.org/wiki/Pseudomonas_phage_JBD30 Pseudomonas phage JBD30] and [https://en.wikipedia.org/wiki/Pseudomonas_virus_D3112 Pseudomonas virus D3112]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZ9 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">csy1, PA14_33330 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB]), csy2, PA14_33320 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB]), csy3, csy1-3, PA14_33310 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB]), JBD30_035 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223260 Pseudomonas phage JBD30]), orf30 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10708 BPD31]), cas6f, csy4, PA14_33300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208963 PSEAB])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz9 OCA], [http://pdbe.org/5uz9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uz9 RCSB], [http://www.ebi.ac.uk/pdbsum/5uz9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uz9 OCA], [https://pdbe.org/5uz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uz9 RCSB], [https://www.ebi.ac.uk/pdbsum/5uz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uz9 ProSAT]</span></td></tr>
</table>
</table>
{{Large structure}}
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CAS6_PSEAB CAS6_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:20829488</ref> <ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CSY3_PSEAB CSY3_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/CSY2_PSEAB CSY2_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Absolutely required for crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  [[http://www.uniprot.org/uniprot/ACR30_BPD31 ACR30_BPD31]] Allows the phage to evade the CRISPR/Cas system type I-F.<ref>PMID:23242138</ref> 
[https://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Genetic conflict between viruses and their hosts drives evolution and genetic innovation. Prokaryotes evolved CRISPR-mediated adaptive immune systems for protection from viral infection, and viruses have evolved diverse anti-CRISPR (Acr) proteins that subvert these immune systems. The adaptive immune system in Pseudomonas aeruginosa (type I-F) relies on a 350 kDa CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease for target degradation. Here, we report the cryo-electron microscopy (cryo-EM) structure of the Csy complex bound to two different Acr proteins, AcrF1 and AcrF2, at an average resolution of 3.4 A. The structure explains the molecular mechanism for immune system suppression, and structure-guided mutations show that the Acr proteins bind to residues essential for crRNA-mediated detection of DNA. Collectively, these data provide a snapshot of an ongoing molecular arms race between viral suppressors and the immune system they target.
 
Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.,Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B Cell. 2017 Mar 23;169(1):47-57.e11. doi: 10.1016/j.cell.2017.03.012. PMID:28340349<ref>PMID:28340349</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[CRISPR subtype I-F|CRISPR subtype I-F]]
<div class="pdbe-citations 5uz9" style="background-color:#fffaf0;"></div>
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Bpd31]]
[[Category: Large Structures]]
[[Category: Pseab]]
[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
[[Category: Pseudomonas phage jbd30]]
[[Category: Pseudomonas phage JBD30]]
[[Category: Bondy-Denomy, J]]
[[Category: Pseudomonas virus D3112]]
[[Category: Carter, J]]
[[Category: Bondy-Denomy J]]
[[Category: Chowdhury, S]]
[[Category: Carter J]]
[[Category: Davidson, A R]]
[[Category: Chowdhury S]]
[[Category: Fischer, E R]]
[[Category: Davidson AR]]
[[Category: Hoffmann, C]]
[[Category: Fischer ER]]
[[Category: Jackson, R N]]
[[Category: Hoffmann C]]
[[Category: Lander, G C]]
[[Category: Jackson RN]]
[[Category: Maxwell, K L]]
[[Category: Lander GC]]
[[Category: Nosaka, L]]
[[Category: Maxwell KL]]
[[Category: Rollins, M F]]
[[Category: Nosaka L]]
[[Category: Wiedenheft, B]]
[[Category: Rollins MF]]
[[Category: Immune system-rna complex]]
[[Category: Wiedenheft B]]
[[Category: Pseudo-helical]]
[[Category: Type 1-f crispr]]

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