4ziz: Difference between revisions

<table><tr><td colspan='2'>[[4ziz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_(strain_bp-1) Thermosynechococcus elongatus (strain bp-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZIZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZIZ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>

<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ziz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ziz OCA], [http://pdbe.org/4ziz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ziz RCSB], [http://www.ebi.ac.uk/pdbsum/4ziz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ziz ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/PHCA_THEEB PHCA_THEEB]] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. [[http://www.uniprot.org/uniprot/PHCB_THEEB PHCB_THEEB]] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex.

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.

 

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.,Fromme R, Ishchenko A, Metz M, Chowdhury SR, Basu S, Boutet S, Fromme P, White TA, Barty A, Spence JC, Weierstall U, Liu W, Cherezov V IUCrJ. 2015 Aug 4;2(Pt 5):545-51. doi: 10.1107/S2052252515013160. eCollection, 2015 Sep 1. PMID:26306196<ref>PMID:26306196</ref>

 

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

[[Category: Thermosynechococcus elongatus]]

[[Category: Barty, A]]

[[Category: Basu, S]]

[[Category: Boutet, S]]

[[Category: Cherezov, V]]

[[Category: Fromme, P]]

[[Category: Fromme, R]]

[[Category: Ishchenko, A]]

[[Category: Liu, W]]

[[Category: Metz, M]]

[[Category: Roy-Chowdhury, S]]

[[Category: Spence, J C.H]]

[[Category: Weierstall, U]]

[[Category: White, T A]]

[[Category: Phycocyanin]]