4zdz: Difference between revisions

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 ==Saccharomyces cerevisiae CYP51 (Lanosterol 14-alpha demethylase) Y140F mutant complexed with fluconazole==
-<StructureSection load='4zdz' size='340' side='right' caption='[[4zdz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='4zdz' size='340' side='right'caption='[[4zdz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zdz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZDZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZDZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zdz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_YJM789 Saccharomyces cerevisiae YJM789]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZDZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k0f|4k0f]], [[4wmz|4wmz]], [[4zdy|4zdy]], [[4ze0|4ze0]], [[4ze1|4ze1]], [[4ze2|4ze2]], [[4ze3|4ze3]], [[4lxj|4lxj]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=TPF:2-(2,4-DIFLUOROPHENYL)-1,3-DI(1H-1,2,4-TRIAZOL-1-YL)PROPAN-2-OL'>TPF</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zdz OCA], [http://pdbe.org/4zdz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zdz RCSB], [http://www.ebi.ac.uk/pdbsum/4zdz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zdz OCA], [https://pdbe.org/4zdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zdz RCSB], [https://www.ebi.ac.uk/pdbsum/4zdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zdz ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/A6ZSR0_YEAS7 A6ZSR0_YEAS7]
-Bitopic integral membrane proteins with a single transmembrane helix play diverse roles in catalysis, cell signaling, and morphogenesis. Complete monospanning protein structures are needed to show how interaction between the transmembrane helix and catalytic domain might influence association with the membrane and function. We report crystal structures of full-length Saccharomyces cerevisiae lanosterol 14alpha-demethylase, a membrane monospanning cytochrome P450 of the CYP51 family that catalyzes the first postcyclization step in ergosterol biosynthesis and is inhibited by triazole drugs. The structures reveal a well-ordered N-terminal amphipathic helix preceding a putative transmembrane helix that would constrain the catalytic domain orientation to lie partly in the lipid bilayer. The structures locate the substrate lanosterol, identify putative substrate and product channels, and reveal constrained interactions with triazole antifungal drugs that are important for drug design and understanding drug resistance.
-Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.,Monk BC, Tomasiak TM, Keniya MV, Huschmann FU, Tyndall JD, O'Connell JD 3rd, Cannon RD, McDonald JG, Rodriguez A, Finer-Moore JS, Stroud RM Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3865-70. doi:, 10.1073/pnas.1324245111. Epub 2014 Feb 3. PMID:24613931<ref>PMID:24613931</ref>
+==See Also==
+*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 4zdz" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Keniya, M V]]
+[[Category: Large Structures]]
-[[Category: Monk, B C]]
+[[Category: Saccharomyces cerevisiae YJM789]]
-[[Category: Sagatova, A]]
+[[Category: Keniya MV]]
-[[Category: Tyndall, J D.A]]
+[[Category: Monk BC]]
-[[Category: Wilson, R K]]
+[[Category: Sagatova A]]
-[[Category: Cyp51]]
+[[Category: Tyndall JDA]]
-[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]
+[[Category: Wilson RK]]
-[[Category: Resistance mutation]]