3c0t: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:3c0t.jpg|left|200px]]
-<!--
+==Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18==
-The line below this paragraph, containing "STRUCTURE_3c0t", creates the "Structure Box" on the page.
+<StructureSection load='3c0t' size='340' side='right'caption='[[3c0t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3c0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C0T FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c0t OCA], [https://pdbe.org/3c0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c0t RCSB], [https://www.ebi.ac.uk/pdbsum/3c0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c0t ProSAT]</span></td></tr>
-{{STRUCTURE_3c0t|  PDB=3c0t  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MED18_SCHPO MED18_SCHPO] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.<ref>PMID:12471453</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/3c0t_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c0t ConSurf].
+<div style="clear:both"></div>
-'''Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18'''
+==See Also==
+*[[Mediator|Mediator]]
+== References ==
-==Overview==
+<references/>
-A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-C0T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0T OCA].
-==Reference==
-Structure-system correlation identifies a gene regulatory Mediator submodule., Lariviere L, Seizl M, van Wageningen S, Rother S, van de Pasch L, Feldmann H, Strasser K, Hahn S, Holstege FC, Cramer P, Genes Dev. 2008 Apr 1;22(7):872-877. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18381891 18381891]
-[[Category: Protein complex]]
 [[Category: Schizosaccharomyces pombe]]
-[[Category: Cramer, P.]]
+[[Category: Cramer P]]
-[[Category: Feldmann, H.]]
+[[Category: Feldmann H]]
-[[Category: Hahn, S.]]
+[[Category: Hahn S]]
-[[Category: Holstege, C P.]]
+[[Category: Holstege CP]]
-[[Category: Lariviere, L.]]
+[[Category: Lariviere L]]
-[[Category: Pasch, L van de.]]
+[[Category: Roether S]]
-[[Category: Roether, S.]]
+[[Category: Seizl M]]
-[[Category: Seizl, M.]]
+[[Category: Straesser K]]
-[[Category: Straesser, K.]]
+[[Category: Van Wageningen S]]
-[[Category: Wageningen, S van.]]
+[[Category: Van de Pasch L]]
-[[Category: Activator]]
-[[Category: Beta barrel]]
-[[Category: Channel]]
-[[Category: Nucleus]]
-[[Category: Protein-protein complex]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:51:25 2008''