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==Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18==
==Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18==
<StructureSection load='3c0t' size='340' side='right' caption='[[3c0t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='3c0t' size='340' side='right'caption='[[3c0t]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3c0t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C0T FirstGlance]. <br>
<table><tr><td colspan='2'>[[3c0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C0T FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hzm|2hzm]], [[2hzs|2hzs]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">med18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356]), med8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c0t OCA], [https://pdbe.org/3c0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c0t RCSB], [https://www.ebi.ac.uk/pdbsum/3c0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c0t ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c0t OCA], [http://pdbe.org/3c0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3c0t RCSB], [http://www.ebi.ac.uk/pdbsum/3c0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3c0t ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MED18_SCHPO MED18_SCHPO]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.<ref>PMID:12471453</ref> [[http://www.uniprot.org/uniprot/MED8_SCHPO MED8_SCHPO]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
[https://www.uniprot.org/uniprot/MED18_SCHPO MED18_SCHPO] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.<ref>PMID:12471453</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c0t ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c0t ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.
Structure-system correlation identifies a gene regulatory Mediator submodule.,Lariviere L, Seizl M, van Wageningen S, Rother S, van de Pasch L, Feldmann H, Strasser K, Hahn S, Holstege FC, Cramer P Genes Dev. 2008 Apr 1;22(7):872-877. PMID:18381891<ref>PMID:18381891</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3c0t" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cbs 356]]
[[Category: Large Structures]]
[[Category: Cramer, P]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Feldmann, H]]
[[Category: Cramer P]]
[[Category: Hahn, S]]
[[Category: Feldmann H]]
[[Category: Holstege, C P]]
[[Category: Hahn S]]
[[Category: Lariviere, L]]
[[Category: Holstege CP]]
[[Category: Pasch, L van de]]
[[Category: Lariviere L]]
[[Category: Roether, S]]
[[Category: Roether S]]
[[Category: Seizl, M]]
[[Category: Seizl M]]
[[Category: Straesser, K]]
[[Category: Straesser K]]
[[Category: Wageningen, S van]]
[[Category: Van Wageningen S]]
[[Category: Activator]]
[[Category: Van de Pasch L]]
[[Category: Beta barrel]]
[[Category: Channel]]
[[Category: Nucleus]]
[[Category: Protein-protein complex]]
[[Category: Transcription]]
[[Category: Transcription regulation]]

Latest revision as of 17:04, 13 March 2024

Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18Structure of the Schizosaccharomyces pombe Mediator subcomplex Med8C/18

Structural highlights

3c0t is a 2 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MED18_SCHPO Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Szilagyi Z, Grallert A, Nemeth N, Sipiczki M. The Schizosaccharomyces pombe genes sep10 and sep11 encode putative general transcriptional regulators involved in multiple cellular processes. Mol Genet Genomics. 2002 Dec;268(4):553-62. Epub 2002 Nov 7. PMID:12471453 doi:http://dx.doi.org/10.1007/s00438-002-0773-3

3c0t, resolution 2.40Å

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