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==Diverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with Cellobiose==
==Diverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with Cellobiose==
<StructureSection load='3azr' size='340' side='right' caption='[[3azr]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
<StructureSection load='3azr' size='340' side='right'caption='[[3azr]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3azr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AZR FirstGlance]. <br>
<table><tr><td colspan='2'>[[3azr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AZR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CBK:4-O-BETA-D-GLUCOPYRANOSYL-ALPHA-D-GLUCOPYRANOSE'>CBK</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3amc|3amc]], [[3amd|3amd]], [[3amg|3amg]], [[3aof|3aof]], [[3azs|3azs]], [[3azt|3azt]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900023:alpha-cellobiose'>PRD_900023</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TM_1751 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3azr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azr OCA], [https://pdbe.org/3azr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3azr RCSB], [https://www.ebi.ac.uk/pdbsum/3azr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3azr ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3azr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3azr OCA], [http://pdbe.org/3azr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3azr RCSB], [http://www.ebi.ac.uk/pdbsum/3azr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3azr ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9X273_THEMA Q9X273_THEMA]
The hyperthermophilic endoglucanase Cel5A from Thermotoga maritima can find applications in lignocellulosic biofuel production, because it catalyzes the hydrolysis of glucan- and mannan-based polysaccharides. Here, we report the crystal structures in apo-form and in complex with three ligands, cellotetraose, cellobiose and mannotriose, at 1.29A to 2.40A resolution. The open carbohydrate-binding cavity which can accommodate oligosaccharide substrates with extensively branched chains explained the dual specificity of the enzyme. Combining our structural information and the previous kinetic data, it is suggested that this enzyme prefers beta-glucosyl and beta-mannosyl moieties at the reducing end and uses two conserved catalytic residues, E253 (nucleophile) and E136 (general acid/base), to hydrolyze the glycosidic bonds. Moreover, our results also suggest that the wide spectrum of Tm_Cel5A substrates might be due to the lack of steric hindrance around the C2-hydroxyl group of the glucose or mannose unit from active-site residues.
 
Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose.,Wu TH, Huang CH, Ko TP, Lai HL, Ma Y, Chen CC, Cheng YS, Liu JR, Guo RT Biochim Biophys Acta. 2011 Dec;1814(12):1832-40. doi:, 10.1016/j.bbapap.2011.07.020. Epub 2011 Aug 4. PMID:21839861<ref>PMID:21839861</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3azr" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Glucanase|Glucanase]]
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cellulase]]
[[Category: Large Structures]]
[[Category: Thema]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Chen, C C]]
[[Category: Chen CC]]
[[Category: Cheng, Y S]]
[[Category: Cheng YS]]
[[Category: Guo, R T]]
[[Category: Guo RT]]
[[Category: Huang, C H]]
[[Category: Huang CH]]
[[Category: Ko, T P]]
[[Category: Ko TP]]
[[Category: Lai, H L]]
[[Category: Lai HL]]
[[Category: Liu, J R]]
[[Category: Liu JR]]
[[Category: Ma, Y]]
[[Category: Ma Y]]
[[Category: Wu, T H]]
[[Category: Wu TH]]
[[Category: Biofuel]]
[[Category: Cellulose]]
[[Category: Hydrolase]]
[[Category: Tim barrel]]

Latest revision as of 17:02, 13 March 2024

Diverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with CellobioseDiverse Substrates Recognition Mechanism Revealed by Thermotoga maritima Cel5A Structures in Complex with Cellobiose

Structural highlights

3azr is a 2 chain structure with sequence from Thermotoga maritima MSB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.71Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9X273_THEMA

See Also

3azr, resolution 1.71Å

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