3a52: Difference between revisions

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 ==Crystal structure of cold-active alkailne phosphatase from psychrophile Shewanella sp.==
-<StructureSection load='3a52' size='340' side='right' caption='[[3a52]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='3a52' size='340' side='right'caption='[[3a52]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3a52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Shewanella Shewanella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A52 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3a52]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_sp._AP1 Shewanella sp. AP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A52 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">scap ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=22 Shewanella])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a52 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a52 RCSB], [http://www.ebi.ac.uk/pdbsum/3a52 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a52 OCA], [https://pdbe.org/3a52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a52 RCSB], [https://www.ebi.ac.uk/pdbsum/3a52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a52 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8RQU7_9GAMM Q8RQU7_9GAMM]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/3a52_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/3a52_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a52 ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-The crystal structure of a cold-active alkaline phosphatase from a psychrophile, Shewanella sp. (SCAP), was solved at 2.2 A. A refined model showed a homodimer with six metal-ligand sites. The arrangement of the catalytic residues resembled those of alkaline phosphatases (APs), suggesting that the reaction mechanism of SCAP was fundamentally identical to those of other APs. SCAP had two distinct structural features: (i) a loop with Arg122 that bound to the phosphate moiety of the substrate suffered no constraints from the linkage to other secondary structures, and (ii) Mg3-ligand His109 was considered to undergo repulsive effect with neighboring Trp228. The local flexibility led by these features might be an important factor in the high catalytic efficiency of SCAP at low temperatures.
-Crystal structure of cold-active alkaline phosphatase from the psychrophile Shewanella sp.,Tsuruta H, Mikami B, Higashi T, Aizono Y Biosci Biotechnol Biochem. 2010 Jan;74(1):69-74. Epub 2010 Jan 7. PMID:20057143<ref>PMID:20057143</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
 ==See Also==
-*[[Alkaline phosphatase|Alkaline phosphatase]]
+*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Shewanella]]
+[[Category: Large Structures]]
-[[Category: Aizono, Y]]
+[[Category: Shewanella sp. AP1]]
-[[Category: Higashi, T]]
+[[Category: Aizono Y]]
-[[Category: Mikami, B]]
+[[Category: Higashi T]]
-[[Category: Tsuruta, H]]
+[[Category: Mikami B]]
-[[Category: Hydrolase]]
+[[Category: Tsuruta H]]
-[[Category: Phosphatase]]