2zj2: Difference between revisions

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New page: '''Unreleased structure''' The entry 2zj2 is ON HOLD until Paper Publication Authors: Oyama, T., Oka, H., Fujikane, R., Ishino, Y., Morikawa, K. Description: Archaeal DNA helicase ''...
 
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'''Unreleased structure'''


The entry 2zj2 is ON HOLD  until Paper Publication
==Archaeal DNA helicase Hjm apo state in form 1==
 
<StructureSection load='2zj2' size='340' side='right'caption='[[2zj2]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
Authors: Oyama, T., Oka, H., Fujikane, R., Ishino, Y., Morikawa, K.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[2zj2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZJ2 FirstGlance]. <br>
Description: Archaeal DNA helicase
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zj2 OCA], [https://pdbe.org/2zj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zj2 RCSB], [https://www.ebi.ac.uk/pdbsum/2zj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zj2 ProSAT]</span></td></tr>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 09:10:12 2008''
</table>
== Function ==
[https://www.uniprot.org/uniprot/HELS_PYRFU HELS_PYRFU] DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks. Unwinds the lagging strand from forked DNA structures in a 3'-5' direction. PCNA, the DNA polymerase sliding clamp subunit, stimulates the helicase activity, and may alter substrate specificity. Unwinds branched DNA (Holliday junctions) in an ATP-dependent fashion; ss- and dsDNA stimulate ATPase to the greatest extent, although it preferentially binds DNA with a single-stranded region. Processes a RecA-mediated recombination intermediate between gapped circular and homologus linear dsDNA.<ref>PMID:15677450</ref> <ref>PMID:16436047</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zj/2zj2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zj2 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Fujikane R]]
[[Category: Ishino Y]]
[[Category: Morikawa K]]
[[Category: Oka H]]
[[Category: Oyama T]]

Latest revision as of 16:57, 13 March 2024

Archaeal DNA helicase Hjm apo state in form 1Archaeal DNA helicase Hjm apo state in form 1

Structural highlights

2zj2 is a 1 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HELS_PYRFU DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks. Unwinds the lagging strand from forked DNA structures in a 3'-5' direction. PCNA, the DNA polymerase sliding clamp subunit, stimulates the helicase activity, and may alter substrate specificity. Unwinds branched DNA (Holliday junctions) in an ATP-dependent fashion; ss- and dsDNA stimulate ATPase to the greatest extent, although it preferentially binds DNA with a single-stranded region. Processes a RecA-mediated recombination intermediate between gapped circular and homologus linear dsDNA.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Fujikane R, Komori K, Shinagawa H, Ishino Y. Identification of a novel helicase activity unwinding branched DNAs from the hyperthermophilic archaeon, Pyrococcus furiosus. J Biol Chem. 2005 Apr 1;280(13):12351-8. Epub 2005 Jan 27. PMID:15677450 doi:http://dx.doi.org/10.1074/jbc.M413417200
  2. ↑ Fujikane R, Shinagawa H, Ishino Y. The archaeal Hjm helicase has recQ-like functions, and may be involved in repair of stalled replication fork. Genes Cells. 2006 Feb;11(2):99-110. PMID:16436047 doi:http://dx.doi.org/10.1111/j.1365-2443.2006.00925.x

2zj2, resolution 2.40Å

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