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==Family 16 Cabohydrate Binding Domain Module 1==
==Family 16 Cabohydrate Binding Domain Module 1==
<StructureSection load='2zew' size='340' side='right' caption='[[2zew]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
<StructureSection load='2zew' size='340' side='right'caption='[[2zew]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2zew]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-17 Atcc baa-17]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZEW FirstGlance]. <br>
<table><tr><td colspan='2'>[[2zew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobius_polysaccharolyticus Caldanaerobius polysaccharolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZEW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zex|2zex]], [[2zey|2zey]], [[2zez|2zez]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">celA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44256 ATCC BAA-17])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zew OCA], [https://pdbe.org/2zew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zew RCSB], [https://www.ebi.ac.uk/pdbsum/2zew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zew ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zew OCA], [http://pdbe.org/2zew PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zew RCSB], [http://www.ebi.ac.uk/pdbsum/2zew PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zew ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9ZA17_9THEO Q9ZA17_9THEO]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zew ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zew ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymes that hydrolyze complex polysaccharides into simple sugars are modular in architecture and consist of single or multiple catalytic domains fused to targeting modules called carbohydrate-binding modules (CBMs). CBMs bind to their ligands with high affinity and increase the efficiency of the catalytic components by targeting the enzymes to its substrate. Here we utilized a multidisciplinary approach to characterize each of the two family 16 carbohydrate-binding domain components of the highly active mannanase from the thermophile Thermoanaerobacterium polysaccharolyticum. These represent the first crystal structures of family 16 CBMs. Calorimetric analysis showed that although these CBMs demonstrate high specificity toward beta-1,4-linked sugars, they can engage both cello- and mannopolysaccharides. To elucidate the molecular basis for this specificity and selectivity, we have determined high resolution crystal structures of each of the two CBMs, as well as of binary complexes of CBM16-1 bound to either mannopentaose or cellopentaose. These results provide detailed molecular insights into ligand recognition and yield a framework for rational engineering experiments designed to expand the natural repertoire of these targeting modules.
Molecular basis for the selectivity and specificity of ligand recognition by the family 16 carbohydrate-binding modules from Thermoanaerobacterium polysaccharolyticum ManA.,Bae B, Ohene-Adjei S, Kocherginskaya S, Mackie RI, Spies MA, Cann IK, Nair SK J Biol Chem. 2008 May 2;283(18):12415-25. Epub 2007 Nov 19. PMID:18025086<ref>PMID:18025086</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zew" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Glucanase|Glucanase]]
*[[Glucanase 3D structures|Glucanase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc baa-17]]
[[Category: Caldanaerobius polysaccharolyticus]]
[[Category: Bae, B]]
[[Category: Large Structures]]
[[Category: Nair, S K]]
[[Category: Bae B]]
[[Category: Carbohydrate binding module]]
[[Category: Nair SK]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]

Latest revision as of 16:57, 13 March 2024

Family 16 Cabohydrate Binding Domain Module 1Family 16 Cabohydrate Binding Domain Module 1

Structural highlights

2zew is a 2 chain structure with sequence from Caldanaerobius polysaccharolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9ZA17_9THEO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2zew, resolution 1.40Å

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