2zew: Difference between revisions

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New page: left|200px<br /><applet load="2zew" size="350" color="white" frame="true" align="right" spinBox="true" caption="2zew, resolution 1.40Å" /> '''Family 16 Cabohydrat...
 
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[[Image:2zew.jpg|left|200px]]<br /><applet load="2zew" size="350" color="white" frame="true" align="right" spinBox="true"
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'''Family 16 Cabohydrate Binding Domain Module 1'''<br />


==Overview==
==Family 16 Cabohydrate Binding Domain Module 1==
Enzymes that hydrolyze complex polysaccharides into simple sugars are modular in architecture and consist of single or multiple catalytic domains fused to targeting modules called carbohydrate binding modules (CBMs). CBMs bind to their ligands with high affinity, and increase the efficiency of the catalytic components by targeting the enzymes to its substrate. Here, we utilized a multi-disciplinary approach to characterize each of the two family 16 carbohydrate binding domain component of the highly active mannanase from the thermophile T. polysaccharolyticum. These represent the first crystal structures of family 16 CBMs. Calorimetric analysis show that while these CBMs demonstrate high specificity towards ss-1,4 linked sugars, they can engage both cello- and manno-polysaccharides. In order to elucidate the molecular basis for this specificity and selectivity, we have determined high resolution crystal structures of each of the two carbohydrate binding modules, as well as of binary complexes of CBM16-1 bound to either mannopentaose or cellopentaose. These results provide detailed molecular insights into ligand recognition and yield a framework for rational engineering experiments designed to expand the natural repertoire of these targeting modules.
<StructureSection load='2zew' size='340' side='right'caption='[[2zew]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2zew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobius_polysaccharolyticus Caldanaerobius polysaccharolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZEW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zew OCA], [https://pdbe.org/2zew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zew RCSB], [https://www.ebi.ac.uk/pdbsum/2zew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zew ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9ZA17_9THEO Q9ZA17_9THEO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/2zew_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zew ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2ZEW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_polysaccharolyticum Thermoanaerobacterium polysaccharolyticum] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Ca+Binding+Site+For+Residue+A+147'>AC1</scene> and <scene name='pdbsite=AC2:Ca+Binding+Site+For+Residue+B+147'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZEW OCA].
*[[Glucanase 3D structures|Glucanase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Molecular basis for the selectivity and specificity of ligand recognition by the family 16 carbohydrate binding modules from thermoanaerobacterium polysaccharolyticum manA., Bae B, Ohene-Adjei S, Kocherginskaya S, Mackie RI, Spies MA, Cann IK, Nair SK, J Biol Chem. 2007 Nov 29;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18025086 18025086]
[[Category: Caldanaerobius polysaccharolyticus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Thermoanaerobacterium polysaccharolyticum]]
[[Category: Bae B]]
[[Category: Bae, B.]]
[[Category: Nair SK]]
[[Category: Nair, S K.]]
[[Category: CA]]
[[Category: carbohydrate binding module]]
[[Category: glycosidase]]
[[Category: hydrolase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar  5 13:23:32 2008''

Latest revision as of 16:57, 13 March 2024

Family 16 Cabohydrate Binding Domain Module 1Family 16 Cabohydrate Binding Domain Module 1

Structural highlights

2zew is a 2 chain structure with sequence from Caldanaerobius polysaccharolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9ZA17_9THEO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2zew, resolution 1.40Å

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