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[[Image:2z8x.gif|left|200px]]


{{Structure
==Crystal structure of extracellular lipase from Pseudomonas sp. MIS38==
|PDB= 2z8x |SIZE=350|CAPTION= <scene name='initialview01'>2z8x</scene>, resolution 1.48&Aring;
<StructureSection load='2z8x' size='340' side='right'caption='[[2z8x]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[2z8x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._MIS38 Pseudomonas sp. MIS38]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8X FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8x OCA], [https://pdbe.org/2z8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8x RCSB], [https://www.ebi.ac.uk/pdbsum/2z8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8x ProSAT]</span></td></tr>
|RELATEDENTRY=[[2z8z|2Z8Z]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8x OCA], [http://www.ebi.ac.uk/pdbsum/2z8x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z8x RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q9RBY1_9PSED Q9RBY1_9PSED]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/2z8x_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z8x ConSurf].
<div style="clear:both"></div>


'''Crystal structure of extracellular lipase from Pseudomonas sp. MIS38'''
==See Also==
 
*[[Lipase 3D Structures|Lipase 3D Structures]]
 
__TOC__
==Overview==
</StructureSection>
The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.
[[Category: Large Structures]]
 
[[Category: Pseudomonas sp. MIS38]]
==About this Structure==
[[Category: Angkawidjaja C]]
2Z8X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._mis38 Pseudomonas sp. mis38]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8X OCA].
[[Category: Kanaya S]]
 
[[Category: Koga Y]]
==Reference==
[[Category: Matsumura H]]
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17923123 17923123]
[[Category: Takano K]]
[[Category: Pseudomonas sp. mis38]]
[[Category: You DJ]]
[[Category: Single protein]]
[[Category: Triacylglycerol lipase]]
[[Category: Angkawidjaja, C.]]
[[Category: Kanaya, S.]]
[[Category: Koga, Y.]]
[[Category: Matsumura, H.]]
[[Category: Takano, K.]]
[[Category: You, D J.]]
[[Category: beta roll]]
[[Category: calcium binding protein]]
[[Category: family i 3 lipase]]
[[Category: hydrolase]]
[[Category: rtx protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:29 2008''

Latest revision as of 16:56, 13 March 2024

Crystal structure of extracellular lipase from Pseudomonas sp. MIS38Crystal structure of extracellular lipase from Pseudomonas sp. MIS38

Structural highlights

2z8x is a 1 chain structure with sequence from Pseudomonas sp. MIS38. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.48Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9RBY1_9PSED

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2z8x, resolution 1.48Å

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