2r1g: Difference between revisions

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[[Image:2r1g.jpg|left|200px]]


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==Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex==
The line below this paragraph, containing "STRUCTURE_2r1g", creates the "Structure Box" on the page.
<SX load='2r1g' size='340' side='right' viewer='molstar' caption='[[2r1g]], [[Resolution|resolution]] 12.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2r1g]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R1G FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r1g OCA], [https://pdbe.org/2r1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r1g RCSB], [https://www.ebi.ac.uk/pdbsum/2r1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r1g ProSAT]</span></td></tr>
{{STRUCTURE_2r1g| PDB=2r1g |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS9_THET2 RS9_THET2] Part of the top of the head of the 30S subunit. The C-terminal region penetrates the head emerging in the P-site where it contacts tRNA (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r1g_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r1g ConSurf].
<div style="clear:both"></div>


'''Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex'''
==See Also==
 
*[[Ribosomal protein S12|Ribosomal protein S12]]
 
*[[Ribosomal protein S13|Ribosomal protein S13]]
==Overview==
*[[Ribosomal protein S9|Ribosomal protein S9]]
Ribosome binding factor A (RbfA) is a bacterial cold shock response protein, required for an efficient processing of the 5' end of the 16S ribosomal RNA (rRNA) during assembly of the small (30S) ribosomal subunit. Here we present a crystal structure of Thermus thermophilus (Tth) RbfA and a three-dimensional cryo-electron microscopic (EM) map of the Tth 30S*RbfA complex. RbfA binds to the 30S subunit in a position overlapping the binding sites of the A and P site tRNAs, and RbfA's functionally important C terminus extends toward the 5' end of the 16S rRNA. In the presence of RbfA, a portion of the 16S rRNA encompassing helix 44, which is known to be directly involved in mRNA decoding and tRNA binding, is displaced. These results shed light on the role played by RbfA during maturation of the 30S subunit, and also indicate how RbfA provides cells with a translational advantage under conditions of cold shock.
__TOC__
 
</SX>
==About this Structure==
[[Category: Large Structures]]
2R1G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R1G OCA].
[[Category: Synthetic construct]]
 
[[Category: Thermus thermophilus HB27]]
==Reference==
[[Category: Agrawal RK]]
Structural aspects of RbfA action during small ribosomal subunit assembly., Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK, Mol Cell. 2007 Nov 9;28(3):434-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17996707 17996707]
[[Category: Booth TM]]
[[Category: Protein complex]]
[[Category: Datta PP]]
[[Category: Thermus thermophilus]]
[[Category: Fucini P]]
[[Category: Agrawal, R K.]]
[[Category: Kaminishi T]]
[[Category: Booth, T M.]]
[[Category: Kawazoe M]]
[[Category: Datta, P P.]]
[[Category: Sharma MR]]
[[Category: Fucini, P.]]
[[Category: Swami NK]]
[[Category: Kaminishi, T.]]
[[Category: Takemoto C]]
[[Category: Kawazoe, M.]]
[[Category: Wilson DN]]
[[Category: Sharma, M R.]]
[[Category: Yokoyama S]]
[[Category: Swami, N K.]]
[[Category: Takemoto, C.]]
[[Category: Wilson, D N.]]
[[Category: Yokoyama, S.]]
[[Category: 30s ribosome maturation protein rbfa]]
[[Category: 30s-rbfa complex]]
[[Category: Antibiotic resistance]]
[[Category: Cold shock response protein rbfa]]
[[Category: Rbfa binding site on the 30]]
[[Category: Ribonucleoprotein]]
[[Category: Ribosomal protein]]
[[Category: Ribosomal protein/rna complex]]
[[Category: Rna-binding]]
[[Category: Rrna-binding]]
[[Category: Trna-binding]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:04:50 2008''

Latest revision as of 16:54, 13 March 2024

Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complexCoordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex

2r1g, resolution 12.50Å

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