2pk0: Difference between revisions

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-[[Image:2pk0.jpg|left|200px]]<br /><applet load="2pk0" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2pk0, resolution 2.65&Aring;" />
-'''Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution'''<br />
-==Overview==
+==Structure of the S. agalactiae serine/threonine phosphatase at 2.65 resolution==
-We solved the crystal structure of Streptococcus agalactiae serine/threonine phosphatase (SaSTP) using a combination of single-wavelength anomalous dispersion phasing and molecular replacement. The overall structure resembles that of previously characterized members of the PPM/PP2C STP family. The asymmetric unit contains four monomers and we observed two novel conformations for the flap domain among them. In one of these conformations, the enzyme binds three metal ions, whereas in the other it binds only two. The three-metal ion structure also has the active site arginine in a novel conformation. The switch between the two- and three-metal ion structures appears to be binding of another monomer to the active site of STP, which promotes binding of the third metal ion. This interaction may mimic the binding of a product complex, especially since the motif binding to the active site contains a serine residue aligning remarkably well with the phosphate found in the human STP structure.
+<StructureSection load='2pk0' size='340' side='right'caption='[[2pk0]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2pk0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_A909 Streptococcus agalactiae A909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PK0 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pk0 OCA], [https://pdbe.org/2pk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2pk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pk0 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8VQA1_STRAG Q8VQA1_STRAG]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pk/2pk0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pk0 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-PK0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PK0 OCA].
+*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structure of Streptococcus agalactiae serine/threonine phosphatase. The subdomain conformation is coupled to the binding of a third metal ion., Rantanen MK, Lehtio L, Rajagopal L, Rubens CE, Goldman A, FEBS J. 2007 Jun;274(12):3128-37. Epub 2007 May 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17521332 17521332]
+[[Category: Large Structures]]
-[[Category: Phosphoprotein phosphatase]]
+[[Category: Streptococcus agalactiae A909]]
-[[Category: Single protein]]
+[[Category: Goldman A]]
-[[Category: Streptococcus agalactiae]]
+[[Category: Lehtio L]]
-[[Category: Goldman, A.]]
+[[Category: Rajagopal L]]
-[[Category: Lehtio, L.]]
+[[Category: Rantanen MK]]
-[[Category: Rajagopal, L.]]
+[[Category: Rubens CE]]
-[[Category: Rantanen, M K.]]
-[[Category: Rubens, C E.]]
-[[Category: CL]]
-[[Category: GOL]]
-[[Category: MG]]
-[[Category: phosphatase]]
-[[Category: serine]]
-[[Category: signaling motif]]
-[[Category: streptococcus agalactiae]]
-[[Category: threonine]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:19 2008''