2gr0: Difference between revisions

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[[Image:2gr0.jpg|left|200px]]


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==Crystal structure of Ferredoxin reductase, BphA4 (oxidized form, NAD+ complex)==
The line below this paragraph, containing "STRUCTURE_2gr0", creates the "Structure Box" on the page.
<StructureSection load='2gr0' size='340' side='right'caption='[[2gr0]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2gr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._KKS102 Pseudomonas sp. KKS102]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GR0 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
{{STRUCTURE_2gr0|  PDB=2gr0  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gr0 OCA], [https://pdbe.org/2gr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gr0 RCSB], [https://www.ebi.ac.uk/pdbsum/2gr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gr0 ProSAT]</span></td></tr>
 
</table>
'''Crystal structure of Ferredoxin reductase, BphA4 (oxidized form, NAD+ complex)'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q52437_PSES1 Q52437_PSES1]  
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The electron transfer system of the biphenyl dioxygenase BphA, which is derived from Acidovorax sp. (formally Pseudomonas sp.) strain KKS102, is composed of an FAD-containing NADH-ferredoxin reductase (BphA4) and a Rieske-type [2Fe-2S] ferredoxin (BphA3). Biochemical studies have suggested that the whole electron transfer process from NADH to BphA3 comprises three consecutive elementary electron-transfer reactions, in which BphA3 and BphA4 interact transiently in a redox-dependent manner. Initially, BphA4 receives two electrons from NADH. The reduced BphA4 then delivers one electron each to the [2Fe-2S] cluster of the two BphA3 molecules through redox-dependent transient interactions. The reduced BphA3 transports the electron to BphA1A2, a terminal oxygenase, to support the activation of dioxygen for biphenyl dihydroxylation. In order to elucidate the molecular mechanisms of the sequential reaction and the redox-dependent interaction between BphA3 and BphA4, we determined the crystal structures of the productive BphA3-BphA4 complex, and of free BphA3 and BphA4 in all the redox states occurring in the catalytic cycle. The crystal structures of these reaction intermediates demonstrated that each elementary electron transfer induces a series of redox-dependent conformational changes in BphA3 and BphA4, which regulate the interaction between them. In addition, the conformational changes induced by the preceding electron transfer seem to induce the next electron transfer. The interplay of electron transfer and induced conformational changes seems to be critical to the sequential electron-transfer reaction from NADH to BphA3.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/2gr0_consurf.spt"</scriptWhenChecked>
2GR0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._kks102 Pseudomonas sp. kks102]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GR0 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin., Senda M, Kishigami S, Kimura S, Fukuda M, Ishida T, Senda T, J Mol Biol. 2007 Oct 19;373(2):382-400. Epub 2007 Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17850818 17850818]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gr0 ConSurf].
[[Category: Pseudomonas sp. kks102]]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Senda, M.]]
</StructureSection>
[[Category: Senda, T.]]
[[Category: Large Structures]]
[[Category: Flavoprotein]]
[[Category: Pseudomonas sp. KKS102]]
[[Category: Oxidoreductase]]
[[Category: Senda M]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:25:17 2008''
[[Category: Senda T]]

Latest revision as of 16:52, 13 March 2024

Crystal structure of Ferredoxin reductase, BphA4 (oxidized form, NAD+ complex)Crystal structure of Ferredoxin reductase, BphA4 (oxidized form, NAD+ complex)

Structural highlights

2gr0 is a 1 chain structure with sequence from Pseudomonas sp. KKS102. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q52437_PSES1

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2gr0, resolution 1.70Å

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OCA
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