2ehb: Difference between revisions

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[[Image:2ehb.jpg|left|200px]]


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==The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3==
The line below this paragraph, containing "STRUCTURE_2ehb", creates the "Structure Box" on the page.
<StructureSection load='2ehb' size='340' side='right'caption='[[2ehb]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2ehb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EHB FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_2ehb|  PDB=2ehb  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ehb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ehb OCA], [https://pdbe.org/2ehb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ehb RCSB], [https://www.ebi.ac.uk/pdbsum/2ehb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ehb ProSAT]</span></td></tr>
 
</table>
'''The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3'''
== Function ==
 
[https://www.uniprot.org/uniprot/CNBL4_ARATH CNBL4_ARATH] Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. May function as positive regulator of salt stress responses. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.<ref>PMID:11006339</ref> <ref>PMID:10725350</ref> <ref>PMID:12034882</ref>
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The plant SOS2 family of protein kinases and their interacting activators, the SOS3 family of calcium-binding proteins, function together in decoding calcium signals elicited by different environmental stimuli. SOS2 is activated by Ca-SOS3 and subsequently phosphorylates the ion transporter SOS1 to bring about cellular ion homeostasis under salt stress. In addition to possessing the kinase activity, members of the SOS2 family of protein kinases can bind to protein phosphatase 2Cs. The crystal structure of the binary complex of Ca-SOS3 with the C-terminal regulatory moiety of SOS2 resolves central questions regarding the dual function of SOS2 as a kinase and a phosphatase-binding protein. A comparison with the structure of unbound SOS3 reveals the basis of the molecular function of this family of kinases and their interacting calcium sensors. Furthermore, our study suggests that the structure of the phosphatase-interaction domain of SOS2 defines a scaffold module conserved from yeast to human.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eh/2ehb_consurf.spt"</scriptWhenChecked>
2EHB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EHB OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3., Sanchez-Barrena MJ, Fujii H, Angulo I, Martinez-Ripoll M, Zhu JK, Albert A, Mol Cell. 2007 May 11;26(3):427-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17499048 17499048]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ehb ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Sanchez-Barrena MJ]]
[[Category: Sanchez-Barrena, M J.]]
[[Category: Protein complex]]
[[Category: Signalling protein/transferase complex]]
[[Category: Sos2 fisl motif]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 02:33:13 2008''

Latest revision as of 16:49, 13 March 2024

The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3

Structural highlights

2ehb is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNBL4_ARATH Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. May function as positive regulator of salt stress responses. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Ishitani M, Liu J, Halfter U, Kim CS, Shi W, Zhu JK. SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. Plant Cell. 2000 Sep;12(9):1667-78. PMID:11006339
  2. ↑ Halfter U, Ishitani M, Zhu JK. The Arabidopsis SOS2 protein kinase physically interacts with and is activated by the calcium-binding protein SOS3. Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3735-40. PMID:10725350 doi:http://dx.doi.org/10.1073/pnas.040577697
  3. ↑ Qiu QS, Guo Y, Dietrich MA, Schumaker KS, Zhu JK. Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8436-41. Epub 2002 May 28. PMID:12034882 doi:http://dx.doi.org/10.1073/pnas.122224699

2ehb, resolution 2.10Å

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