2d7f: Difference between revisions

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-[[Image:2d7f.gif|left|200px]]<br /><applet load="2d7f" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2d7f, resolution 2.31&Aring;" />
-'''Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid'''<br />
-==Overview==
+==Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid==
-BACKGROUND: Lectins are mainly described as simple carbohydrate-binding proteins. Previous studies have tried to identify other binding sites, which possible recognize plant hormones, secondary metabolites, and isolated amino acid residues. We report the crystal structure of a lectin isolated from Canavalia gladiata seeds (CGL), describing a new binding pocket, which may be related to pathogen resistance activity in ConA-like lectins; a site where a non-protein amino-acid, alpha-aminobutyric acid (Abu), is bound. RESULTS: The overall structure of native CGL and complexed with alpha-methyl-mannoside and Abu have been refined at 2.3 A and 2.31 A resolution, respectively. Analysis of the electron density maps of the CGL structure shows clearly the presence of Abu, which was confirmed by mass spectrometry. CONCLUSION: The presence of Abu in a plant lectin structure strongly indicates the ability of lectins on carrying secondary metabolites. Comparison of the amino acids composing the site with other legume lectins revealed that this site is conserved, providing an evidence of the biological relevance of this site. This new action of lectins strengthens their role in defense mechanisms in plants.
+<StructureSection load='2d7f' size='340' side='right'caption='[[2d7f]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2d7f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_gladiata Canavalia gladiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D7F FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d7f OCA], [https://pdbe.org/2d7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d7f RCSB], [https://www.ebi.ac.uk/pdbsum/2d7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d7f ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CONA_CANGL CONA_CANGL] Glucose/D-mannose/rhamnose specific lectin. Has hemagglutinating activity towards rabbit erythrocytes. Has mitogenic activity towards murine splenocytes that is inhibited by glucose. Inhibits HIV-1 reverse transcriptase with an IC(50) of 35uM. Has a potent antiproliferative activity against L1210 leukemia cells in vitro that is not inhibited by glucose. Inhibits translation in cell-free rabbit reticulocyte system with an IC(50) of 2.08uM. Lacks anti-fungal activity against M.arachidicola, B.cenera and F.oxysporum.<ref>PMID:15935326</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/2d7f_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d7f ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-D7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_gladiata Canavalia gladiata] with <scene name='pdbligand=MMA:'>MMA</scene>, <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ABA:'>ABA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7F OCA].
+*[[Concanavalin 3D structures|Concanavalin 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structure of a lectin from Canavalia gladiata seeds: new structural insights for old molecules., Delatorre P, Rocha BA, Souza EP, Oliveira TM, Bezerra GA, Moreno FB, Freitas BT, Santi-Gadelha T, Sampaio AH, Azevedo WF Jr, Cavada BS, BMC Struct Biol. 2007 Aug 2;7:52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17683532 17683532]
+__TOC__
+</StructureSection>
 [[Category: Canavalia gladiata]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Cavada, B S.]]
+[[Category: Azevedo Jr WF]]
-[[Category: Delatorre, P.]]
+[[Category: Cavada BS]]
-[[Category: Freitas, B T.]]
+[[Category: Delatorre P]]
-[[Category: Jr., W F.Azevedo.]]
+[[Category: Freitas BT]]
-[[Category: Moreno, F B.B M.]]
+[[Category: Moreno FBBM]]
-[[Category: Rocha, B A.M.]]
+[[Category: Rocha BAM]]
-[[Category: Sampaio, A H.]]
+[[Category: Sampaio AH]]
-[[Category: Souza, E P.]]
+[[Category: Souza EP]]
-[[Category: ABA]]
-[[Category: CA]]
-[[Category: MMA]]
-[[Category: MN]]
-[[Category: diocleinae]]
-[[Category: lectin]]
-[[Category: nonprotein amino acid]]
-[[Category: plant protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:55:55 2008''