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[[Image:2d7f.png|left|200px]]


{{STRUCTURE_2d7f|  PDB=2d7f  |  SCENE=  }}
==Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid==
 
<StructureSection load='2d7f' size='340' side='right'caption='[[2d7f]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
===Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[2d7f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_gladiata Canavalia gladiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D7F FirstGlance]. <br>
{{ABSTRACT_PUBMED_17683532}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
==About this Structure==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d7f OCA], [https://pdbe.org/2d7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d7f RCSB], [https://www.ebi.ac.uk/pdbsum/2d7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d7f ProSAT]</span></td></tr>
[[2d7f]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_gladiata Canavalia gladiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D7F OCA].  
</table>
== Function ==
[https://www.uniprot.org/uniprot/CONA_CANGL CONA_CANGL] Glucose/D-mannose/rhamnose specific lectin. Has hemagglutinating activity towards rabbit erythrocytes. Has mitogenic activity towards murine splenocytes that is inhibited by glucose. Inhibits HIV-1 reverse transcriptase with an IC(50) of 35uM. Has a potent antiproliferative activity against L1210 leukemia cells in vitro that is not inhibited by glucose. Inhibits translation in cell-free rabbit reticulocyte system with an IC(50) of 2.08uM. Lacks anti-fungal activity against M.arachidicola, B.cenera and F.oxysporum.<ref>PMID:15935326</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d7/2d7f_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d7f ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Concanavalin A|Concanavalin A]]
*[[Concanavalin 3D structures|Concanavalin 3D structures]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:017683532</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Canavalia gladiata]]
[[Category: Canavalia gladiata]]
[[Category: Azevedo, W F.]]
[[Category: Large Structures]]
[[Category: Cavada, B S.]]
[[Category: Azevedo Jr WF]]
[[Category: Delatorre, P.]]
[[Category: Cavada BS]]
[[Category: Freitas, B T.]]
[[Category: Delatorre P]]
[[Category: Moreno, F B.B M.]]
[[Category: Freitas BT]]
[[Category: Rocha, B A.M.]]
[[Category: Moreno FBBM]]
[[Category: Sampaio, A H.]]
[[Category: Rocha BAM]]
[[Category: Souza, E P.]]
[[Category: Sampaio AH]]
[[Category: Diocleinae]]
[[Category: Souza EP]]
[[Category: Lectin]]
[[Category: Nonprotein amino acid]]
[[Category: Plant protein]]

Latest revision as of 16:46, 13 March 2024

Crystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acidCrystal structure of A lectin from canavalia gladiata seeds complexed with alpha-methyl-mannoside and alpha-aminobutyric acid

Structural highlights

2d7f is a 4 chain structure with sequence from Canavalia gladiata. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.31Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CONA_CANGL Glucose/D-mannose/rhamnose specific lectin. Has hemagglutinating activity towards rabbit erythrocytes. Has mitogenic activity towards murine splenocytes that is inhibited by glucose. Inhibits HIV-1 reverse transcriptase with an IC(50) of 35uM. Has a potent antiproliferative activity against L1210 leukemia cells in vitro that is not inhibited by glucose. Inhibits translation in cell-free rabbit reticulocyte system with an IC(50) of 2.08uM. Lacks anti-fungal activity against M.arachidicola, B.cenera and F.oxysporum.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Wong JH, Ng TB. Isolation and characterization of a glucose/mannose/rhamnose-specific lectin from the knife bean Canavalia gladiata. Arch Biochem Biophys. 2005 Jul 1;439(1):91-8. PMID:15935326 doi:http://dx.doi.org/S0003-9861(05)00186-4

2d7f, resolution 2.31Å

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OCA
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