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[[Image:2d38.gif|left|200px]]<br /><applet load="2d38" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2d38, resolution 2.05&Aring;" />
'''The Crystal Structure of Flavin Reductase HpaC complexed with NADP+'''<br />


==Overview==
==The Crystal Structure of Flavin Reductase HpaC complexed with NADP+==
4-Hydroxyphenylacetate (4-HPA) is oxidized as an energy source by two component enzymes, the large component (HpaB) and the small component (HpaC). HpaB is a 4-HPA monooxygenase that utilizes FADH(2) supplied by a flavin reductase HpaC. We determined the crystal structure of HpaC (ST0723) from the aerobic thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7 in its three states [NAD(P)(+)-free, NAD(+)-bound, and NADP(+)-bound]. HpaC exists as a homodimer, and each monomer was found to contain an FMN. HpaC preferred FMN to FAD because there was not enough space to accommodate the AMP moiety of FAD in its flavin-binding site. The most striking difference between the NAD(P)(+)-free and the NAD(+)/NADP(+)-bound structures was observed in the N-terminal helix. The N-terminal helices in the NAD(+)/NADP(+)-bound structures rotated ca. 20 degrees relative to the NAD(P)(+)-free structure. The bound NAD(+) has a compact folded conformation with nearly parallel stacking rings of nicotinamide and adenine. The nicotinamide of NAD(+) stacked the isoalloxazine ring of FMN so that NADH could directly transfer hydride. The bound NADP(+) also had a compact conformation but was bound in a reverse direction, which was not suitable for hydride transfer.
<StructureSection load='2d38' size='340' side='right'caption='[[2d38]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2d38]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D38 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D38 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d38 OCA], [https://pdbe.org/2d38 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d38 RCSB], [https://www.ebi.ac.uk/pdbsum/2d38 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d38 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q974C9_SULTO Q974C9_SULTO]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d38_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d38 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2D38 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii_str._7 Sulfolobus tokodaii str. 7] with <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D38 OCA].
*[[Flavin reductase|Flavin reductase]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)(+)(-)free, NAD(+)(-)bound, and NADP(+)(-)bound., Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M, Biochemistry. 2006 Apr 25;45(16):5103-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16618099 16618099]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Sulfurisphaera tokodaii str. 7]]
[[Category: Sulfolobus tokodaii str. 7]]
[[Category: Kamo M]]
[[Category: Kamo, M.]]
[[Category: Kudo N]]
[[Category: Kudo, N.]]
[[Category: Lee WC]]
[[Category: Lee, W C.]]
[[Category: Nagata K]]
[[Category: Nagata, K.]]
[[Category: Okai M]]
[[Category: Okai, M.]]
[[Category: Tanokura M]]
[[Category: Tanokura, M.]]
[[Category: FMN]]
[[Category: NAP]]
[[Category: flavin reductase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:54:56 2008''

Latest revision as of 16:46, 13 March 2024

The Crystal Structure of Flavin Reductase HpaC complexed with NADP+The Crystal Structure of Flavin Reductase HpaC complexed with NADP+

Structural highlights

2d38 is a 1 chain structure with sequence from Sulfurisphaera tokodaii str. 7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.05Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q974C9_SULTO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2d38, resolution 2.05Å

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