2d2s: Difference between revisions

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New page: left|200px<br /><applet load="2d2s" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d2s, resolution 2.85Å" /> '''Crystal Structure of...
 
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[[Image:2d2s.jpg|left|200px]]<br /><applet load="2d2s" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2d2s, resolution 2.85&Aring;" />
'''Crystal Structure of the Exo84p C-terminal Domains'''<br />


==Overview==
==Crystal Structure of the Exo84p C-terminal Domains==
The exocyst is a large complex that is required for tethering vesicles at, the final stages of the exocytic pathway in all eukaryotes. Here we, present the structures of the Exo70p subunit of this complex and of the, C-terminal domains of Exo84p, at 2.0-A and 2.85-A resolution, respectively. Exo70p forms a 160-A-long rod with a novel fold composed of, contiguous alpha-helical bundles. The Exo84p C terminus also forms a long, rod (80 A), which unexpectedly has the same fold as the Exo70p N terminus., Our structural results and our experimental observations concerning the, interaction between Exo70p and other exocyst subunits or Rho3p GTPase are, consistent with an architecture wherein exocyst subunits are composed of, mostly helical modules strung together into long rods.
<StructureSection load='2d2s' size='340' side='right'caption='[[2d2s]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2d2s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D2S FirstGlance]. <br>
2D2S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2D2S OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d2s OCA], [https://pdbe.org/2d2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d2s RCSB], [https://www.ebi.ac.uk/pdbsum/2d2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d2s ProSAT]</span></td></tr>
==Reference==
</table>
The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif., Dong G, Hutagalung AH, Fu C, Novick P, Reinisch KM, Nat Struct Mol Biol. 2005 Dec;12(12):1094-100. Epub 2005 Oct 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16249794 16249794]
== Function ==
[https://www.uniprot.org/uniprot/EXO84_YEAST EXO84_YEAST] Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome.<ref>PMID:10438536</ref> <ref>PMID:11425851</ref> <ref>PMID:15788396</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/2d2s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d2s ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Dong G]]
[[Category: Dong, G.]]
[[Category: Fu C]]
[[Category: Fu, C.]]
[[Category: Hutagalung AH]]
[[Category: Hutagalung, A.H.]]
[[Category: Novick P]]
[[Category: Novick, P.]]
[[Category: Reinisch KM]]
[[Category: Reinisch, K.M.]]
[[Category: exo84p]]
[[Category: exocyst]]
[[Category: tethering complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:24:33 2007''

Latest revision as of 16:45, 13 March 2024

Crystal Structure of the Exo84p C-terminal DomainsCrystal Structure of the Exo84p C-terminal Domains

Structural highlights

2d2s is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.85Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EXO84_YEAST Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in both the assembly of the exocyst and the polarization of this complex to specific sites of the plasma membrane for exocytosis and to the budding site. Also involved in assembly of the spliceosome.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Guo W, Grant A, Novick P. Exo84p is an exocyst protein essential for secretion. J Biol Chem. 1999 Aug 13;274(33):23558-64. PMID:10438536
  2. ↑ Awasthi S, Palmer R, Castro M, Mobarak CD, Ruby SW. New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem. 2001 Aug 17;276(33):31004-15. Epub 2001 Jun 25. PMID:11425851 doi:http://dx.doi.org/10.1074/jbc.M100022200
  3. ↑ Zhang X, Zajac A, Zhang J, Wang P, Li M, Murray J, TerBush D, Guo W. The critical role of Exo84p in the organization and polarized localization of the exocyst complex. J Biol Chem. 2005 May 27;280(21):20356-64. Epub 2005 Mar 23. PMID:15788396 doi:http://dx.doi.org/M500511200

2d2s, resolution 2.85Å

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