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[[Image:2avf.gif|left|200px]]


{{Structure
==Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes==
|PDB= 2avf |SIZE=350|CAPTION= <scene name='initialview01'>2avf</scene>, resolution 2.6&Aring;
<StructureSection load='2avf' size='340' side='right'caption='[[2avf]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
<table><tr><td colspan='2'>[[2avf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AVF FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2avf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avf OCA], [https://pdbe.org/2avf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2avf RCSB], [https://www.ebi.ac.uk/pdbsum/2avf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2avf ProSAT]</span></td></tr>
|RELATEDENTRY=[[1rzp|1RZP]], [[1rzq|1RZQ]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2avf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avf OCA], [http://www.ebi.ac.uk/pdbsum/2avf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2avf RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/NIR_ACHCY NIR_ACHCY]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/2avf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2avf ConSurf].
<div style="clear:both"></div>


'''Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes'''
==See Also==
 
*[[Nitrite reductase 3D structures|Nitrite reductase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Monoclinic crystal structure of C-terminal desundecapeptide nitrite reductase (NiRc-11) from Achromobacter cycloclastes was determined at 2.6A. NiRc-11 exists as a loose trimer in the crystal. Deletion of 11 residues eliminates all intersubunit hydrogen bonds mediated by the C-terminal tail. The rigid irregular coil 105-112, which constitutes part of the sidewall of the active site pocket, undergoes conformational changes and becomes highly flexible in NiRc-11. Correspondingly, the linker segments between the two copper sites 95-100 and 135-136 are partly relaxed in conformation, which leads to disrupted active site microenvironments responsible for the activity loss and spectral change of NiRc-11. Comparison with the native structure revealed a bulky residue Met331 fastened by hydrogen bonding, which may play a direct role in keeping the right copper site geometry by protruding its side chain against the irregular coil 105-112. Sequence alignment showed that the bulky residue is conserved at position 331, indicating an equal importance of C-terminal segment in other copper-containing nitrite reductases.
 
==About this Structure==
2AVF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVF OCA].
 
==Reference==
Crystal structure of C-terminal desundecapeptide nitrite reductase from Achromobacter cycloclastes., Li HT, Chang T, Chang WC, Chen CJ, Liu MY, Gui LL, Zhang JP, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Dec 30;338(4):1935-42. Epub 2005 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16293231 16293231]
[[Category: Achromobacter cycloclastes]]
[[Category: Achromobacter cycloclastes]]
[[Category: Nitrite reductase (NO-forming)]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: An XM]]
[[Category: An, X M.]]
[[Category: Chang T]]
[[Category: Chang, T.]]
[[Category: Chang WC]]
[[Category: Chang, W C.]]
[[Category: Chang WR]]
[[Category: Chang, W R.]]
[[Category: Chen CJ]]
[[Category: Chen, C J.]]
[[Category: Gui LL]]
[[Category: Gui, L L.]]
[[Category: Li HT]]
[[Category: Li, H T.]]
[[Category: Liu MY]]
[[Category: Liu, M Y.]]
[[Category: Zhang JP]]
[[Category: Zhang, J P.]]
[[Category: beta barrel trimer]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:57:50 2008''

Latest revision as of 16:42, 13 March 2024

Crystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastesCrystal Structure of C-terminal Desundecapeptide Nitrite Reductase from Achromobacter cycloclastes

Structural highlights

2avf is a 6 chain structure with sequence from Achromobacter cycloclastes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NIR_ACHCY

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2avf, resolution 2.60Å

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OCA
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