1xi5: Difference between revisions

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New page: left|200px<br /><applet load="1xi5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xi5" /> '''Clathrin D6 coat with auxilin J-domain'''<br...
 
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[[Image:1xi5.gif|left|200px]]<br /><applet load="1xi5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xi5" />
'''Clathrin D6 coat with auxilin J-domain'''<br />


==Overview==
==Clathrin D6 coat with auxilin J-domain==
Clathrin-coated pits invaginate from specific membrane compartments and, pinch off as coated vesicles. These vesicles then uncoat rapidly once, released. The Hsc70 molecular chaperone effects the uncoating reaction, and is guided to appropriate locations on clathrin lattices by the, J-domain-containing co-chaperone molecule auxilin. This raises the, question of how a local event such as ATP hydrolysis by Hsc70 can catalyse, a global disassembly. Here, we have used electron cryomicroscopy to, determine 12-A-resolution structures of in-vitro-assembled clathrin coats, in association with a carboxy-terminal fragment of auxilin that contains, both the clathrin-binding region and the J domain. We have located the, auxilin fragment by computing differences between these structures and, those lacking auxilin (described in an accompanying paper). Auxilin binds, within the clathrin lattice near contacts between an inward-projecting, C-terminal helical tripod and the crossing of two 'ankle' segments; it, also contacts the terminal domain of yet another clathrin 'leg'. It, therefore recruits Hsc70 to the neighbourhood of a set of critical, interactions. Auxilin binding produces a local change in heavy-chain, contacts, creating a detectable global distortion of the clathrin coat. We, propose a mechanism by which local destabilization of the lattice promotes, general uncoating.
<SX load='1xi5' size='340' side='right' viewer='molstar' caption='[[1xi5]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xi5]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XI5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xi5 OCA], [https://pdbe.org/1xi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xi5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xi5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLH1_BOVIN CLH1_BOVIN] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xi/1xi5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xi5 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1XI5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XI5 OCA].
*[[Clathrin 3D structures|Clathrin 3D structures]]
 
__TOC__
==Reference==
</SX>
Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating., Fotin A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T, Nature. 2004 Dec 2;432(7017):649-53. Epub 2004 Oct 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15502813 15502813]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Cheng, Y.]]
[[Category: Cheng Y]]
[[Category: Fotin, A.]]
[[Category: Fotin A]]
[[Category: Grigorieff, N.]]
[[Category: Grigorieff N]]
[[Category: Harrison, S.C.]]
[[Category: Harrison SC]]
[[Category: Kirchhausen, T.]]
[[Category: Kirchhausen T]]
[[Category: Walz, T.]]
[[Category: Walz T]]
[[Category: alpha-zig-zag]]
[[Category: beta-propeller]]
[[Category: clathrin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:05:13 2007''

Latest revision as of 16:36, 13 March 2024

Clathrin D6 coat with auxilin J-domainClathrin D6 coat with auxilin J-domain

1xi5, resolution 12.00Å

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