1xi5: Difference between revisions

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<SX load='1xi5' size='340' side='right' viewer='molstar' caption='[[1xi5]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
<SX load='1xi5' size='340' side='right' viewer='molstar' caption='[[1xi5]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1xi5]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XI5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XI5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1xi5]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XI5 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xi4|1xi4]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xi5 OCA], [http://pdbe.org/1xi5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xi5 RCSB], [http://www.ebi.ac.uk/pdbsum/1xi5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xi5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xi5 OCA], [https://pdbe.org/1xi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xi5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xi5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CLH1_BOVIN CLH1_BOVIN]] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. [[http://www.uniprot.org/uniprot/AUXI_BOVIN AUXI_BOVIN]] Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles.<ref>PMID:15502813</ref> 
[https://www.uniprot.org/uniprot/CLH1_BOVIN CLH1_BOVIN] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xi5 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xi5 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Clathrin-coated pits invaginate from specific membrane compartments and pinch off as coated vesicles. These vesicles then uncoat rapidly once released. The Hsc70 molecular chaperone effects the uncoating reaction, and is guided to appropriate locations on clathrin lattices by the J-domain-containing co-chaperone molecule auxilin. This raises the question of how a local event such as ATP hydrolysis by Hsc70 can catalyse a global disassembly. Here, we have used electron cryomicroscopy to determine 12-A-resolution structures of in-vitro-assembled clathrin coats in association with a carboxy-terminal fragment of auxilin that contains both the clathrin-binding region and the J domain. We have located the auxilin fragment by computing differences between these structures and those lacking auxilin (described in an accompanying paper). Auxilin binds within the clathrin lattice near contacts between an inward-projecting C-terminal helical tripod and the crossing of two 'ankle' segments; it also contacts the terminal domain of yet another clathrin 'leg'. It therefore recruits Hsc70 to the neighbourhood of a set of critical interactions. Auxilin binding produces a local change in heavy-chain contacts, creating a detectable global distortion of the clathrin coat. We propose a mechanism by which local destabilization of the lattice promotes general uncoating.
Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating.,Fotin A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T Nature. 2004 Dec 2;432(7017):649-53. Epub 2004 Oct 24. PMID:15502813<ref>PMID:15502813</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1xi5" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Clathrin 3D structures|Clathrin 3D structures]]
*[[Clathrin 3D structures|Clathrin 3D structures]]
== References ==
<references/>
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__TOC__
</SX>
</SX>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Cheng, Y]]
[[Category: Cheng Y]]
[[Category: Fotin, A]]
[[Category: Fotin A]]
[[Category: Grigorieff, N]]
[[Category: Grigorieff N]]
[[Category: Harrison, S C]]
[[Category: Harrison SC]]
[[Category: Kirchhausen, T]]
[[Category: Kirchhausen T]]
[[Category: Walz, T]]
[[Category: Walz T]]
[[Category: Alpha-zig-zag]]
[[Category: Beta-propeller]]
[[Category: Clathrin]]
[[Category: Endocytosis-exocytosis complex]]

Latest revision as of 16:36, 13 March 2024

Clathrin D6 coat with auxilin J-domainClathrin D6 coat with auxilin J-domain

1xi5, resolution 12.00Å

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