1xi4: Difference between revisions

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New page: left|200px<br /> <applet load="1xi4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xi4" /> '''Clathrin D6 Coat'''<br /> ==Overview== Cla...
 
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[[Image:1xi4.gif|left|200px]]<br />
<applet load="1xi4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xi4" />
'''Clathrin D6 Coat'''<br />


==Overview==
==Clathrin D6 Coat==
Clathrin-coated vesicles are important vehicles of membrane traffic in, cells. We report the structure of a clathrin lattice at subnanometre, resolution, obtained from electron cryomicroscopy of coats assembled in, vitro. We trace most of the 1,675-residue clathrin heavy chain by fitting, known crystal structures of two segments, and homology models of the rest, into the electron microscopy density map. We also define the position of, the central helical segment of the light chain. A helical tripod, the, carboxy-terminal parts of three heavy chains, projects inward from the, vertex of each three-legged clathrin triskelion, linking that vertex to, 'ankles' of triskelions centred two vertices away. Analysis of coats with, distinct diameters shows an invariant pattern of contacts in the, neighbourhood of each vertex, with more variable interactions along the, extended parts of the triskelion 'legs'. These invariant local, interactions appear to stabilize the lattice, allowing assembly and, uncoating to be controlled by events at a few specific sites.
<SX load='1xi4' size='340' side='right' viewer='molstar' caption='[[1xi4]], [[Resolution|resolution]] 7.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xi4]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. The April 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Clathrin''  by Graham T. Johnson and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_4 10.2210/rcsb_pdb/mom_2007_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XI4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xi4 OCA], [https://pdbe.org/1xi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xi4 RCSB], [https://www.ebi.ac.uk/pdbsum/1xi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xi4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLH1_BOVIN CLH1_BOVIN] Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xi/1xi4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xi4 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1XI4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. The following page contains interesting information on the relation of 1XI4 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb88_1.html Clathrin]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XI4 OCA].
*[[Clathrin 3D structures|Clathrin 3D structures]]
 
__TOC__
==Reference==
</SX>
Molecular model for a complete clathrin lattice from electron cryomicroscopy., Fotin A, Cheng Y, Sliz P, Grigorieff N, Harrison SC, Kirchhausen T, Walz T, Nature. 2004 Dec 2;432(7017):573-9. Epub 2004 Oct 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15502812 15502812]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Clathrin]]
[[Category: Clathrin]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Cheng, Y.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Fotin, A.]]
[[Category: Cheng Y]]
[[Category: Grigorieff, N.]]
[[Category: Fotin A]]
[[Category: Harrison, S.C.]]
[[Category: Grigorieff N]]
[[Category: Kirchhausen, T.]]
[[Category: Harrison SC]]
[[Category: Sliz, P.]]
[[Category: Kirchhausen T]]
[[Category: Walz, T.]]
[[Category: Sliz P]]
[[Category: alpha-zig-zag]]
[[Category: Walz T]]
[[Category: beta-propeller]]
[[Category: clathrin]]
 
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Latest revision as of 16:36, 13 March 2024

Clathrin D6 CoatClathrin D6 Coat

1xi4, resolution 7.90Å

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